Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kuhlmann, A.U.; Bremer, E.
    Osmotically regulated synthesis of the compatible solute ectoine in Bacillus pasteurii and related Bacillus spp. (2002), Appl. Environ. Microbiol., 68, 772-783.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.108 additional information high osmolarity induces the enzyme expression, requirements Virgibacillus pantothenticus
4.2.1.108 additional information high osmolarity induces the enzyme expression, requirements Alkalihalobacillus alcalophilus
4.2.1.108 additional information high osmolarity induces the enzyme expression, requirements Sporosarcina psychrophila
4.2.1.108 additional information high osmolarity induces the enzyme expression, requirements Sporosarcina pasteurii
4.2.1.108 additional information high osmolarity induces the enzyme expression, requirements Virgibacillus salexigens
4.2.1.108 additional information high osmolarity induces the enzyme expression, requirements Chromohalobacter salexigens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.178
-
 Sporosarcina pasteurii
4.2.1.108 gene ectC encoded in the ectABC gene cluster, functional expression in Escherichia coli Chromohalobacter salexigens
4.2.1.108 gene ectC, encoded in the ectABC gene cluster, sequence determination and analysis, phylogenetic analysis, DNA sequence comparison, expression in Escherichia coli Sporosarcina pasteurii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.108 NaCl required for activity Sporosarcina pasteurii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate Sporosarcina pasteurii together EctA, EctB and EctC constitute the ectoine biosynthetic pathway. ectABC genes are genetically organized as an operon whose expression is strongly enhanced when the osmolarity of the growth medium is raised CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate Virgibacillus pantothenticus final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant L-ectoine + H2O
-
 ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate Alkalihalobacillus alcalophilus final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant L-ectoine + H2O
-
 ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate Sporosarcina psychrophila final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant L-ectoine + H2O
-
 ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate Sporosarcina pasteurii final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant L-ectoine + H2O
-
 ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate Virgibacillus salexigens final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant L-ectoine + H2O
-
 ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate Chromohalobacter salexigens final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant L-ectoine + H2O
-
 ?
4.2.1.108 additional information Virgibacillus pantothenticus phylogenetic analysis ?
-
 ?
4.2.1.108 additional information Alkalihalobacillus alcalophilus phylogenetic analysis ?
-
 ?
4.2.1.108 additional information Sporosarcina psychrophila phylogenetic analysis ?
-
 ?
4.2.1.108 additional information Sporosarcina pasteurii phylogenetic analysis ?
-
 ?
4.2.1.108 additional information Virgibacillus salexigens phylogenetic analysis ?
-
 ?
4.2.1.108 additional information Chromohalobacter salexigens phylogenetic analysis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.178 Sporosarcina pasteurii
-
-
-
4.2.1.108 Alkalihalobacillus alcalophilus
-
 strain DSM 485
-
4.2.1.108 Chromohalobacter salexigens Q9ZEU6 formerly Halomonas elongata, strain DSM 3043, gene ectC encoded in the ectABC gene cluster
-
4.2.1.108 no activity in Aneurinibacillus aneurinilyticus
-
 formerly Bacillus aneurinilyticus
-
4.2.1.108 no activity in Aneurinibacillus aneurinilyticus DSM 5562
-
 formerly Bacillus aneurinilyticus
-
4.2.1.108 no activity in Bacillus cereus
-
 strain DSM31
-
4.2.1.108 no activity in Bacillus cereus DSM 31
-
 strain DSM31
-
4.2.1.108 no activity in Bacillus circulans
-
 strain DSM 9
-
4.2.1.108 no activity in Bacillus licheniformis
-
 strain DSM 13
-
4.2.1.108 no activity in Bacillus megaterium
-
 strain DSM 32
-
4.2.1.108 no activity in Bacillus subtilis
-
-
-
4.2.1.108 no activity in Bacillus subtilis JH642
-
-
-
4.2.1.108 no activity in Bacillus thuringiensis
-
 strain DSM 2046
-
4.2.1.108 no activity in Paenibacillus polymyxa
-
 strain DSM 36
-
4.2.1.108 Sporosarcina pasteurii Q9AP33 strain DSM 33, gene ectC encoded in the ectABC gene cluster
-
4.2.1.108 Sporosarcina psychrophila
-
 formerly Bacillus psychrophilus, strain DSM 3
-
4.2.1.108 Virgibacillus pantothenticus
-
 formerl Bacillus pantothenticus, strain DSM 26
-
4.2.1.108 Virgibacillus salexigens
-
 formerly Bacillus salexigens, strain DSM 11438
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.178 acetyl-CoA + L-2,4-diaminobutanoate together EctA, EctB and EctC constitute the ectoine biosynthetic pathway. ectABC genes are genetically organized as an operon whose expression is strongly enhanced when the osmolarity of the growth medium is raised Sporosarcina pasteurii CoA + N4-acetyl-L-2,4-diaminobutanoate
-
 ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant Virgibacillus pantothenticus L-ectoine + H2O
-
 ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant Alkalihalobacillus alcalophilus L-ectoine + H2O
-
 ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant Sporosarcina psychrophila L-ectoine + H2O
-
 ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant Sporosarcina pasteurii L-ectoine + H2O
-
 ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant Virgibacillus salexigens L-ectoine + H2O
-
 ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate final step in L-ectoine biosynthesis, L-ectoine acts as an osmoprotectant Chromohalobacter salexigens L-ectoine + H2O
-
 ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid Sporosarcina psychrophila L-ectoine + H2O NMR identification ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid Sporosarcina pasteurii L-ectoine + H2O NMR identification ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid Virgibacillus pantothenticus L-ectoine + H2O NMR product identification ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid Alkalihalobacillus alcalophilus L-ectoine + H2O NMR product identification ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid Virgibacillus salexigens L-ectoine + H2O NMR product identification ?
4.2.1.108 4-N-acetyl-L-2,4-diaminobutanoate i.e. 1,4,5,6-tetrahydro-2-methyl-4-pyrimidine carboxylic acid Chromohalobacter salexigens L-ectoine + H2O NMR product identification ?
4.2.1.108 additional information phylogenetic analysis Virgibacillus pantothenticus ?
-
 ?
4.2.1.108 additional information phylogenetic analysis Alkalihalobacillus alcalophilus ?
-
 ?
4.2.1.108 additional information phylogenetic analysis Sporosarcina psychrophila ?
-
 ?
4.2.1.108 additional information phylogenetic analysis Sporosarcina pasteurii ?
-
 ?
4.2.1.108 additional information phylogenetic analysis Virgibacillus salexigens ?
-
 ?
4.2.1.108 additional information phylogenetic analysis Chromohalobacter salexigens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.178 EctA
-
 Sporosarcina pasteurii