EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.20 | additional information | no inhibition by phosphate, the activity in higher in phosphate buffer compared to HEPES buffer | Cricetulus griseus | |
3.1.3.5 | 2,3-bisphosphoglycerate | - |
Homo sapiens | |
3.1.3.5 | ATP | - |
Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.20 | expression in Escherichia coli | Cricetulus griseus |
3.1.3.5 | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.20 | 0.0032 | - |
adenosine | recombinant enzyme, pH 7.3, 37°C, phosphate buffered saline | Cricetulus griseus | |
2.7.1.20 | 0.54 | - |
ribavirin | recombinant enzyme, pH 7.3, 37°C, phosphate buffered saline | Cricetulus griseus | |
2.7.1.20 | 7.7 | - |
viramidine | recombinant enzyme, pH 9.0, 37°C, phosphate buffered saline | Cricetulus griseus | |
2.7.1.20 | 7.8 | - |
ribavirin | recombinant enzyme, pH 7.3, 37°C, 0.1 M HEPES buffer | Cricetulus griseus | |
2.7.1.20 | 16 | - |
viramidine | recombinant enzyme, pH 7.3, 37°C, phosphate buffered saline | Cricetulus griseus | |
3.1.3.5 | additional information | - |
additional information | Km-value for viramidine is above 0.5 mM | Homo sapiens | |
3.1.3.5 | 0.024 | - |
ribavirin | presence of 2 mM ATP, phosphate-buffered saline, pH 7.4 | Homo sapiens | |
3.1.3.5 | 0.047 | - |
ribavirin | presence of 0.4 mM 2,3-bisphosphoglycerate, Tris-buffer, pH 7.4 | Homo sapiens | |
3.1.3.5 | 0.088 | - |
ribavirin | presence of 0.4 mM 2,3-bisphosphoglycerate, phosphate-buffered saline, pH 7.4 | Homo sapiens | |
3.1.3.5 | 0.095 | - |
ribavirin | presence of 2 mM ATP, Tris-buffer, pH 7.4 | Homo sapiens | |
3.1.3.5 | 1.8 | - |
Inosine | presence of 0.4 mM 2,3-bisphosphoglycerate, Tris-buffer, pH 7.4 | Homo sapiens | |
3.1.3.5 | 2.17 | - |
Inosine | presence of 2 mM ATP, Tris-buffer, pH 7.4 | Homo sapiens |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.20 | Mg2+ | at 1.5 mM MgCl2 | Cricetulus griseus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.20 | ATP + adenosine | Cricetulus griseus | - |
ADP + AMP | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.20 | Cricetulus griseus | - |
- |
- |
3.1.3.5 | Homo sapiens | P49902 | isoform cytosolic 5'-nucleotidase II, expression in Escherichia coli | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.20 | recombinant enzyme from Escherichia coli to homogeneity | Cricetulus griseus |
3.1.3.5 | recombinant enzyme | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.20 | CHO cell | - |
Cricetulus griseus | - |
3.1.3.5 | placenta | - |
Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.20 | additional information | - |
- |
Cricetulus griseus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.20 | ATP + adenosine | - |
Cricetulus griseus | ADP + AMP | - |
? | |
2.7.1.20 | ATP + ribavirin | very low activity | Cricetulus griseus | ADP + ribavirin 5'-phosphate | - |
? | |
2.7.1.20 | ATP + viramidine | very low activity | Cricetulus griseus | ADP + viramidine 5'-phosphate | - |
? | |
3.1.3.5 | inosine + 5'-IMP | - |
Homo sapiens | 5'-IMP + inosine | - |
? | |
3.1.3.5 | ribavirin + 5'-IMP | better substrate than viramidine | Homo sapiens | ? | - |
? | |
3.1.3.5 | viramidine + 5'-IMP | worse substrate than ribavirin | Homo sapiens | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.20 | 37 | - |
assay at | Cricetulus griseus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.20 | 0.16 | - |
viramidine | recombinant enzyme, pH 7.3, 37°C, phosphate buffered saline | Cricetulus griseus | |
2.7.1.20 | 0.26 | - |
viramidine | recombinant enzyme, pH 9.0, 37°C, phosphate buffered saline | Cricetulus griseus | |
2.7.1.20 | 0.62 | - |
ribavirin | recombinant enzyme, pH 7.3, 37°C, 0.1 M HEPES buffer | Cricetulus griseus | |
2.7.1.20 | 1.8 | - |
ribavirin | recombinant enzyme, pH 7.3, 37°C, phosphate buffered saline | Cricetulus griseus | |
2.7.1.20 | 13 | - |
adenosine | recombinant enzyme, pH 7.3, 37°C, phosphate buffered saline | Cricetulus griseus | |
3.1.3.5 | 0.023 | - |
ribavirin | presence of 2 mM ATP, phosphate-buffered saline, pH 7.4 | Homo sapiens | |
3.1.3.5 | 0.027 | - |
ribavirin | presence of 0.4 mM 2,3-bisphosphoglycerate, Tris-buffer, pH 7.4 | Homo sapiens | |
3.1.3.5 | 0.067 | - |
ribavirin | presence of 0.4 mM 2,3-bisphosphoglycerate, PBS-buffer, pH 7.4 | Homo sapiens | |
3.1.3.5 | 0.15 | - |
ribavirin | presence of 2 mM ATP, Tris-buffer, pH 7.4 | Homo sapiens | |
3.1.3.5 | 19 | - |
Inosine | presence of 0.4 mM 2,3-bisphosphoglycerate, Tris-buffer, pH 7.4 | Homo sapiens | |
3.1.3.5 | 22 | - |
Inosine | presence of 2 mM ATP, Tris-buffer, pH 7.4 | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.20 | 7.3 | - |
assay at | Cricetulus griseus |