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Literature summary extracted from

  • Wu, J.Z.; Larson, G.; Walker, H.; Shim, J.H.; Hong, Z.
    Phosphorylation of ribavirin and viramidine by adenosine kinase and cytosolic 5'-nucleotidase II: Implications for ribavirin metabolism in erythrocytes (2005), Antimicrob. Agents Chemother., 49, 2164-2171.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.20 additional information no inhibition by phosphate, the activity in higher in phosphate buffer compared to HEPES buffer Cricetulus griseus
3.1.3.5 2,3-bisphosphoglycerate
-
Homo sapiens
3.1.3.5 ATP
-
Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.20 expression in Escherichia coli Cricetulus griseus
3.1.3.5
-
Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.20 0.0032
-
adenosine recombinant enzyme, pH 7.3, 37°C, phosphate buffered saline Cricetulus griseus
2.7.1.20 0.54
-
ribavirin recombinant enzyme, pH 7.3, 37°C, phosphate buffered saline Cricetulus griseus
2.7.1.20 7.7
-
viramidine recombinant enzyme, pH 9.0, 37°C, phosphate buffered saline Cricetulus griseus
2.7.1.20 7.8
-
ribavirin recombinant enzyme, pH 7.3, 37°C, 0.1 M HEPES buffer Cricetulus griseus
2.7.1.20 16
-
viramidine recombinant enzyme, pH 7.3, 37°C, phosphate buffered saline Cricetulus griseus
3.1.3.5 additional information
-
additional information Km-value for viramidine is above 0.5 mM Homo sapiens
3.1.3.5 0.024
-
ribavirin presence of 2 mM ATP, phosphate-buffered saline, pH 7.4 Homo sapiens
3.1.3.5 0.047
-
ribavirin presence of 0.4 mM 2,3-bisphosphoglycerate, Tris-buffer, pH 7.4 Homo sapiens
3.1.3.5 0.088
-
ribavirin presence of 0.4 mM 2,3-bisphosphoglycerate, phosphate-buffered saline, pH 7.4 Homo sapiens
3.1.3.5 0.095
-
ribavirin presence of 2 mM ATP, Tris-buffer, pH 7.4 Homo sapiens
3.1.3.5 1.8
-
Inosine presence of 0.4 mM 2,3-bisphosphoglycerate, Tris-buffer, pH 7.4 Homo sapiens
3.1.3.5 2.17
-
Inosine presence of 2 mM ATP, Tris-buffer, pH 7.4 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.20 Mg2+ at 1.5 mM MgCl2 Cricetulus griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.20 ATP + adenosine Cricetulus griseus
-
ADP + AMP
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.20 Cricetulus griseus
-
-
-
3.1.3.5 Homo sapiens P49902 isoform cytosolic 5'-nucleotidase II, expression in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.20 recombinant enzyme from Escherichia coli to homogeneity Cricetulus griseus
3.1.3.5 recombinant enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.20 CHO cell
-
Cricetulus griseus
-
3.1.3.5 placenta
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.20 additional information
-
-
Cricetulus griseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.20 ATP + adenosine
-
Cricetulus griseus ADP + AMP
-
?
2.7.1.20 ATP + ribavirin very low activity Cricetulus griseus ADP + ribavirin 5'-phosphate
-
?
2.7.1.20 ATP + viramidine very low activity Cricetulus griseus ADP + viramidine 5'-phosphate
-
?
3.1.3.5 inosine + 5'-IMP
-
Homo sapiens 5'-IMP + inosine
-
?
3.1.3.5 ribavirin + 5'-IMP better substrate than viramidine Homo sapiens ?
-
?
3.1.3.5 viramidine + 5'-IMP worse substrate than ribavirin Homo sapiens ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.20 37
-
assay at Cricetulus griseus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.20 0.16
-
viramidine recombinant enzyme, pH 7.3, 37°C, phosphate buffered saline Cricetulus griseus
2.7.1.20 0.26
-
viramidine recombinant enzyme, pH 9.0, 37°C, phosphate buffered saline Cricetulus griseus
2.7.1.20 0.62
-
ribavirin recombinant enzyme, pH 7.3, 37°C, 0.1 M HEPES buffer Cricetulus griseus
2.7.1.20 1.8
-
ribavirin recombinant enzyme, pH 7.3, 37°C, phosphate buffered saline Cricetulus griseus
2.7.1.20 13
-
adenosine recombinant enzyme, pH 7.3, 37°C, phosphate buffered saline Cricetulus griseus
3.1.3.5 0.023
-
ribavirin presence of 2 mM ATP, phosphate-buffered saline, pH 7.4 Homo sapiens
3.1.3.5 0.027
-
ribavirin presence of 0.4 mM 2,3-bisphosphoglycerate, Tris-buffer, pH 7.4 Homo sapiens
3.1.3.5 0.067
-
ribavirin presence of 0.4 mM 2,3-bisphosphoglycerate, PBS-buffer, pH 7.4 Homo sapiens
3.1.3.5 0.15
-
ribavirin presence of 2 mM ATP, Tris-buffer, pH 7.4 Homo sapiens
3.1.3.5 19
-
Inosine presence of 0.4 mM 2,3-bisphosphoglycerate, Tris-buffer, pH 7.4 Homo sapiens
3.1.3.5 22
-
Inosine presence of 2 mM ATP, Tris-buffer, pH 7.4 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.20 7.3
-
assay at Cricetulus griseus