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Literature summary extracted from

  • Yamada, H.; Ishii, Y.; Noguchi, Y.; Miura, T.; Mori, T.; Saito, Y.
    Protein engineering of a cephalosporin C acylase (1996), Ann. N. Y. Acad. Sci., 799, 74-81.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.93 synthesis immobilized mutant enzyme M269Y/C305S may be a promising enzyme in one-step enzymatic production of 7-aminocephalosporanate from cephalosporin C Pseudomonas sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.5.1.93 C102S mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C Pseudomonas sp.
3.5.1.93 C199S mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C Pseudomonas sp.
3.5.1.93 C277S mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C Pseudomonas sp.
3.5.1.93 C305S mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C. Expression in Escherichia coli is 2-3fold higher than that of the wild-type enzyme Pseudomonas sp.
3.5.1.93 C391S mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C Pseudomonas sp.
3.5.1.93 C493S mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C Pseudomonas sp.
3.5.1.93 C496S mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C Pseudomonas sp.
3.5.1.93 C748S mutant enzyme retains activity towards glutaryl-7-aminocephalosporanic acid and cephalosporin C Pseudomonas sp.
3.5.1.93 M269Y/C305S 1.6fold higher activity with cephalosporin C than wild-type enzyme Pseudomonas sp.

General Stability

EC Number General Stability Organism
3.5.1.93 immobilized mutant enzyme M269Y/C305S is effective in more than 60 cycles for the production of 7-aminocephalosporanate Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.93 PCMB
-
 Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.93 Pseudomonas sp.
-
 N176
-

Storage Stability

EC Number Storage Stability Organism
3.5.1.93 25°C, 4 weeks, mutant enzyme C305S loses 25% of its activity, mutant enzyme M269Y/C305S loses 25% of its activity Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.93 cephalosporin C + H2O
-
 Pseudomonas sp. cephalosporanic acid + 2-amino-5-hydroxypentanoate
-
 ?
3.5.1.93 glutaryl-7-aminocephalosporanic acid + H2O
-
 Pseudomonas sp. 7-aminocephalosporanate + glutarate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.93 cephalosporin C acylase
-
 Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.1.93 0.102
-
 PCMB
-
 Pseudomonas sp.