BRENDA - Enzyme Database

Activation of PI(4)P 5-kinase by small G proteins

Kanaho, Y.; Miyazaki, H.; Yamazaki, M.; Adv. Enzyme Regul. 43, 107-119 (2003)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.68
small G protein ARF
ARF activation absolutely requires the presence of phosphatidic acid, which cannot be substituted by phosphatidylserine, phosphatidylethanolamine, or lysophosphatidic acid, but by competitive phosphatidylcholine, reconstitution of the recombinant either isozyme alpha, beta, or gamma with recombinant class II and III ARFs, ARF5 and ARF6, respectively, as well as myristoylated class I ARF in presence of GTPgammaS or GDP, and phosphatidic acid activates the isozymes
Mus musculus
2.7.1.68
small G protein RHO
activates the enzyme in neurits, crucial in cell shapig together with the enzyme, overview
Mus musculus
2.7.1.68
small G proteins
-
Bos taurus
2.7.1.68
small G proteins ARF
ARF activation absolutely requires the presence of phosphatidic acid, which cannot be substituted by phosphatidylserine, phosphatidylethanolamine, or lysophosphatidic acid, but by competitive phosphatidylcholine
Homo sapiens
2.7.1.68
small G proteins ARF
ARF activation absolutely requires the presence of phosphatidic acid, which cannot be substituted by phosphatidylserine, phosphatidylethanolamine, or lysophosphatidic acid, but by competitive phosphatidylcholine
Rattus norvegicus
Cloned(Commentary)
EC Number
Commentary
Organism
2.7.1.68
functional expression in HeLa cells as GFP-fusion protein, translocation to the plasma membrane by coexpression of ARF6 upon stimulation by EGF, coexpression of ARF6 mutant N122I inhibits the membrane ruffling, overview
Homo sapiens
2.7.1.68
overexpression of mutant D266A in N1E-115 cells, expression of isozyme alpha in COS-7 cells, expression of isozyme alpha in Escherichia coli
Mus musculus
Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.68
D266A
site-directed mtagenesis, mutation of the putative ATP and 1-phosphatidyl-1D-myo-inositol 4-phosphate binding site, cells form extremely extended bipolar neurits even in presence of serum, failed to interfere with cell flattening and neurite extension
Mus musculus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.68
additional information
GTPgammaS shows poor or no activation of the brain cytosol enzyme but acts synergistically with phosphatidic acid
Bos taurus
2.7.1.68
phosphatidic acid
2-3fold activation of the brain cytosol enzyme, 30fold enhancement of the activation by GTPgammaS, even higher activation by addition of recombinant mouse isozyme alpha
Bos taurus
2.7.1.68
small G proteins ARF
ARF activation absolutely requires the presence of phosphatidic acid, which cannot be substituted by phosphatidylserine, phosphatidylethanolamine, or lysophophatidic acid, but by competitive phosphatidylcholine, proteins ARF1 and ARF3, from brain cytosol, purification and identification, the 21 kDa G-proteins are activated by GTPgammaS, not by GDP, and activate themselves the PI(4)P 5-kinase
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.68
cytosol
-
Bos taurus
5829
-
2.7.1.68
cytosol
-
Homo sapiens
5829
-
2.7.1.68
cytosol
-
Rattus norvegicus
5829
-
2.7.1.68
cytosol
-
Mus musculus
5829
-
2.7.1.68
Golgi apparatus
ARF recruits brain cytosol enzyme from the cytosol to the Golgi complex in an in vitro system
Bos taurus
5794
-
2.7.1.68
plasma membrane
translocation to the plasma membrane of isozyme beta is dependent on ARF6 and EGF, and is completely inhibited by the recombinant GTP-binding-deficient ARF6 mutant N122I
Homo sapiens
5886
-
2.7.1.68
plasma membrane
translocation to the plasma membrane of isozyme alpha is dependent on ARF6 and EGF
Mus musculus
5886
-
2.7.1.68
plasma membrane
translocation to the plasma membrane of isozyme alpha is dependent on ARF6 and EGF
Rattus norvegicus
5886
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.68
Mg2+
-
Bos taurus
2.7.1.68
Mg2+
-
Rattus norvegicus
2.7.1.68
Mg2+
-
Homo sapiens
2.7.1.68
Mg2+
-
Mus musculus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
Mus musculus
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiologic functions, overview
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
Bos taurus
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiologic functions, overview
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
Homo sapiens
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiological functions, overview
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
Rattus norvegicus
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiological functions, overview
-
?
2.7.1.68
additional information
Homo sapiens
ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
?
-
-
-
2.7.1.68
additional information
Rattus norvegicus
ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
?
-
-
-
2.7.1.68
additional information
Bos taurus
ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
?
-
-
-
2.7.1.68
additional information
Mus musculus
enzyme is important in cell signaling and cell shaping, overview, the enzyme is essential for regulation of neurite modeling, ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.68
Bos taurus
-
-
-
2.7.1.68
Homo sapiens
-
3 isozymes alpha, beta, and gamma
-
2.7.1.68
Mus musculus
-
3 isozymes alpha, beta, and gamma
-
2.7.1.68
Rattus norvegicus
-
3 isozymes alpha, beta, and gamma
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.68
brain
-
Bos taurus
-
2.7.1.68
fibroblast
-
Mus musculus
-
2.7.1.68
N1E-115 cell
neuroblastoma cell
Mus musculus
-
2.7.1.68
neuroblastoma cell
-
Mus musculus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
660641
Mus musculus
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
660641
Homo sapiens
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
660641
Rattus norvegicus
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
660641
Bos taurus
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
660641
Mus musculus
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiologic functions, overview
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
660641
Bos taurus
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiologic functions, overview
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
660641
Homo sapiens
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiological functions, overview
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
660641
Rattus norvegicus
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiological functions, overview
-
-
?
2.7.1.68
additional information
ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
660641
Homo sapiens
?
-
-
-
-
2.7.1.68
additional information
ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
660641
Rattus norvegicus
?
-
-
-
-
2.7.1.68
additional information
ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
660641
Bos taurus
?
-
-
-
-
2.7.1.68
additional information
enzyme is important in cell signaling and cell shaping, overview, the enzyme is essential for regulation of neurite modeling, ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
660641
Mus musculus
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.68
More
the enzyme possesses a central socalled kinase core domain containing the activation loop which is responsible for substrate specificity and subcellular targeting of the enzyme
Bos taurus
2.7.1.68
More
the enzyme possesses a central so-called kinase core domain containing the activation loop which is responsible for substrate specificity and subcellular targeting of the enzyme
Homo sapiens
2.7.1.68
More
the enzyme possesses a central so-called kinase core domain containing the activation loop which is responsible for substrate specificity and subcellular targeting of the enzyme
Mus musculus
2.7.1.68
More
the enzyme possesses a central socalled kinase core domain containing the activation loop which is responsible for substrate specificity and subcellular targeting of the enzyme
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.68
ATP
-
Bos taurus
2.7.1.68
ATP
-
Rattus norvegicus
2.7.1.68
ATP
-
Homo sapiens
2.7.1.68
ATP
-
Mus musculus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.68
small G protein ARF
ARF activation absolutely requires the presence of phosphatidic acid, which cannot be substituted by phosphatidylserine, phosphatidylethanolamine, or lysophosphatidic acid, but by competitive phosphatidylcholine, reconstitution of the recombinant either isozyme alpha, beta, or gamma with recombinant class II and III ARFs, ARF5 and ARF6, respectively, as well as myristoylated class I ARF in presence of GTPgammaS or GDP, and phosphatidic acid activates the isozymes
Mus musculus
2.7.1.68
small G protein RHO
activates the enzyme in neurits, crucial in cell shapig together with the enzyme, overview
Mus musculus
2.7.1.68
small G proteins
-
Bos taurus
2.7.1.68
small G proteins ARF
ARF activation absolutely requires the presence of phosphatidic acid, which cannot be substituted by phosphatidylserine, phosphatidylethanolamine, or lysophosphatidic acid, but by competitive phosphatidylcholine
Homo sapiens
2.7.1.68
small G proteins ARF
ARF activation absolutely requires the presence of phosphatidic acid, which cannot be substituted by phosphatidylserine, phosphatidylethanolamine, or lysophosphatidic acid, but by competitive phosphatidylcholine
Rattus norvegicus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.68
functional expression in HeLa cells as GFP-fusion protein, translocation to the plasma membrane by coexpression of ARF6 upon stimulation by EGF, coexpression of ARF6 mutant N122I inhibits the membrane ruffling, overview
Homo sapiens
2.7.1.68
overexpression of mutant D266A in N1E-115 cells, expression of isozyme alpha in COS-7 cells, expression of isozyme alpha in Escherichia coli
Mus musculus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.7.1.68
ATP
-
Bos taurus
2.7.1.68
ATP
-
Rattus norvegicus
2.7.1.68
ATP
-
Homo sapiens
2.7.1.68
ATP
-
Mus musculus
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.1.68
D266A
site-directed mtagenesis, mutation of the putative ATP and 1-phosphatidyl-1D-myo-inositol 4-phosphate binding site, cells form extremely extended bipolar neurits even in presence of serum, failed to interfere with cell flattening and neurite extension
Mus musculus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.68
additional information
GTPgammaS shows poor or no activation of the brain cytosol enzyme but acts synergistically with phosphatidic acid
Bos taurus
2.7.1.68
phosphatidic acid
2-3fold activation of the brain cytosol enzyme, 30fold enhancement of the activation by GTPgammaS, even higher activation by addition of recombinant mouse isozyme alpha
Bos taurus
2.7.1.68
small G proteins ARF
ARF activation absolutely requires the presence of phosphatidic acid, which cannot be substituted by phosphatidylserine, phosphatidylethanolamine, or lysophophatidic acid, but by competitive phosphatidylcholine, proteins ARF1 and ARF3, from brain cytosol, purification and identification, the 21 kDa G-proteins are activated by GTPgammaS, not by GDP, and activate themselves the PI(4)P 5-kinase
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.68
cytosol
-
Bos taurus
5829
-
2.7.1.68
cytosol
-
Homo sapiens
5829
-
2.7.1.68
cytosol
-
Rattus norvegicus
5829
-
2.7.1.68
cytosol
-
Mus musculus
5829
-
2.7.1.68
Golgi apparatus
ARF recruits brain cytosol enzyme from the cytosol to the Golgi complex in an in vitro system
Bos taurus
5794
-
2.7.1.68
plasma membrane
translocation to the plasma membrane of isozyme beta is dependent on ARF6 and EGF, and is completely inhibited by the recombinant GTP-binding-deficient ARF6 mutant N122I
Homo sapiens
5886
-
2.7.1.68
plasma membrane
translocation to the plasma membrane of isozyme alpha is dependent on ARF6 and EGF
Mus musculus
5886
-
2.7.1.68
plasma membrane
translocation to the plasma membrane of isozyme alpha is dependent on ARF6 and EGF
Rattus norvegicus
5886
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.68
Mg2+
-
Bos taurus
2.7.1.68
Mg2+
-
Rattus norvegicus
2.7.1.68
Mg2+
-
Homo sapiens
2.7.1.68
Mg2+
-
Mus musculus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
Mus musculus
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiologic functions, overview
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
Bos taurus
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiologic functions, overview
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
Homo sapiens
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiological functions, overview
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
Rattus norvegicus
-
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiological functions, overview
-
?
2.7.1.68
additional information
Homo sapiens
ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
?
-
-
-
2.7.1.68
additional information
Rattus norvegicus
ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
?
-
-
-
2.7.1.68
additional information
Bos taurus
ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
?
-
-
-
2.7.1.68
additional information
Mus musculus
enzyme is important in cell signaling and cell shaping, overview, the enzyme is essential for regulation of neurite modeling, ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.68
brain
-
Bos taurus
-
2.7.1.68
fibroblast
-
Mus musculus
-
2.7.1.68
N1E-115 cell
neuroblastoma cell
Mus musculus
-
2.7.1.68
neuroblastoma cell
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
660641
Mus musculus
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
660641
Homo sapiens
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
660641
Rattus norvegicus
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
660641
Bos taurus
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
660641
Mus musculus
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiologic functions, overview
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
660641
Bos taurus
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiologic functions, overview
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
660641
Homo sapiens
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiological functions, overview
-
-
?
2.7.1.68
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
660641
Rattus norvegicus
ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate has several important physiological functions, overview
-
-
?
2.7.1.68
additional information
ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
660641
Homo sapiens
?
-
-
-
-
2.7.1.68
additional information
ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
660641
Rattus norvegicus
?
-
-
-
-
2.7.1.68
additional information
ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
660641
Bos taurus
?
-
-
-
-
2.7.1.68
additional information
enzyme is important in cell signaling and cell shaping, overview, the enzyme is essential for regulation of neurite modeling, ARF6-dependent enzyme activation plays an important role in signal transduction of membrane ruffling formation through 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
660641
Mus musculus
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.68
More
the enzyme possesses a central socalled kinase core domain containing the activation loop which is responsible for substrate specificity and subcellular targeting of the enzyme
Bos taurus
2.7.1.68
More
the enzyme possesses a central so-called kinase core domain containing the activation loop which is responsible for substrate specificity and subcellular targeting of the enzyme
Homo sapiens
2.7.1.68
More
the enzyme possesses a central so-called kinase core domain containing the activation loop which is responsible for substrate specificity and subcellular targeting of the enzyme
Mus musculus
2.7.1.68
More
the enzyme possesses a central socalled kinase core domain containing the activation loop which is responsible for substrate specificity and subcellular targeting of the enzyme
Rattus norvegicus