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Literature summary extracted from

  • Das, S.; Kumar, P.; Bhor, V.; Surolia, A.; Vijayan, M.
    Expression, purification, crystallization and preliminary X-ray crystallographic analysis of pantothenate kinase from Mycobacterium tuberculosis (2005), Acta Crystallogr. Sect. F, F61, 65-67.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.33 DNA and amino acid sequence determination and analysis, expression of the His-tagged in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.33 purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, 0.003 ml protein solution containing 6 mg/ml protein, 0.1 M Tris-HCl, pH 8.0, 0.15 M NaCl, 5% v/v glycerol and 0.001 M 2-mercaptoethanol, mixed 0.001 ml of precipitant solution containing 10-15% w/v PEG 8000, 0.05-0.1 M NaOAc, 0.05 NaCl, and 0.1 M cacodylate, pH 6.5, room temperature, equilibration against 0.4 ml precipitant solution, 3-7 days, 2 different crystal forms, X-ray diffraction structure determination and analysis at 2.5 A and 2.9 A resolution, respectively, molecular replacement calculations Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.33 37500
-
x * 37500, recombinant enzyme, SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate Mycobacterium tuberculosis first step in coenzyme A biosynthesis ADP + (R)-4'-phosphopantothenate
-
?
2.7.1.33 ATP + (R)-pantothenate Mycobacterium tuberculosis H37Rv first step in coenzyme A biosynthesis ADP + (R)-4'-phosphopantothenate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Mycobacterium tuberculosis
-
-
-
2.7.1.33 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.33 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate first step in coenzyme A biosynthesis Mycobacterium tuberculosis ADP + (R)-4'-phosphopantothenate
-
?
2.7.1.33 ATP + (R)-pantothenate i.e. vitamin B5 Mycobacterium tuberculosis ADP + (R)-4'-phosphopantothenate
-
?
2.7.1.33 ATP + (R)-pantothenate first step in coenzyme A biosynthesis Mycobacterium tuberculosis H37Rv ADP + (R)-4'-phosphopantothenate
-
?
2.7.1.33 ATP + (R)-pantothenate i.e. vitamin B5 Mycobacterium tuberculosis H37Rv ADP + (R)-4'-phosphopantothenate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.33 ? x * 37500, recombinant enzyme, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.33 PanK
-
Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.33 ATP
-
Mycobacterium tuberculosis