EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.33 | DNA and amino acid sequence determination and analysis, expression of the His-tagged in Escherichia coli strain BL21(DE3) | Mycobacterium tuberculosis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.1.33 | purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, 0.003 ml protein solution containing 6 mg/ml protein, 0.1 M Tris-HCl, pH 8.0, 0.15 M NaCl, 5% v/v glycerol and 0.001 M 2-mercaptoethanol, mixed 0.001 ml of precipitant solution containing 10-15% w/v PEG 8000, 0.05-0.1 M NaOAc, 0.05 NaCl, and 0.1 M cacodylate, pH 6.5, room temperature, equilibration against 0.4 ml precipitant solution, 3-7 days, 2 different crystal forms, X-ray diffraction structure determination and analysis at 2.5 A and 2.9 A resolution, respectively, molecular replacement calculations | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.33 | 37500 | - |
x * 37500, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.33 | ATP + (R)-pantothenate | Mycobacterium tuberculosis | first step in coenzyme A biosynthesis | ADP + (R)-4'-phosphopantothenate | - |
? | |
2.7.1.33 | ATP + (R)-pantothenate | Mycobacterium tuberculosis H37Rv | first step in coenzyme A biosynthesis | ADP + (R)-4'-phosphopantothenate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.33 | Mycobacterium tuberculosis | - |
- |
- |
2.7.1.33 | Mycobacterium tuberculosis H37Rv | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.33 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.33 | ATP + (R)-pantothenate | first step in coenzyme A biosynthesis | Mycobacterium tuberculosis | ADP + (R)-4'-phosphopantothenate | - |
? | |
2.7.1.33 | ATP + (R)-pantothenate | i.e. vitamin B5 | Mycobacterium tuberculosis | ADP + (R)-4'-phosphopantothenate | - |
? | |
2.7.1.33 | ATP + (R)-pantothenate | first step in coenzyme A biosynthesis | Mycobacterium tuberculosis H37Rv | ADP + (R)-4'-phosphopantothenate | - |
? | |
2.7.1.33 | ATP + (R)-pantothenate | i.e. vitamin B5 | Mycobacterium tuberculosis H37Rv | ADP + (R)-4'-phosphopantothenate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.33 | ? | x * 37500, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.33 | PanK | - |
Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.33 | ATP | - |
Mycobacterium tuberculosis |