Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Blewett, A.M.; Lloyd, A.J.; Echalier, A.; Fulop, V.; Dowson, C.G.; Bugg, T.D.; Roper, D.I.
    Expression, purification, crystallization and preliminary characterization of uridine 5'-diphospho-N-acetylmuramoyl L-alanyl-D-glutamate:lysine ligase (MurE) from Streptococcus pneumoniae 110K/70 (2004), Acta Crystallogr. Sect. D, 60, 359-361.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.7 cloned into the high-expression plasmid pET21b and overexpressed in Escherichia coli BL21 (DE3) Star Streptococcus pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.2.7 crystals are obtained by the hanging-drop vapour-diffusion method at 291 K from solutions containing 25%(w/v) polyethylene glycol 2000 monomethylether, 0.2 M potassium thiocyanate, 0.1 M MES pH 6.5 in the presence of uridine 5'-diphospho-N-acetylmuramoyl alanyl glutamate (UDP-MurNAc-l-Ala-d-Glu) with and without 5'-adenylyl imidophosphate (AMP-PNP), a non-hydrolysable analogue of ATP. Crystals grown in the presence of two ligands belong to space group P1, with unit-cell parameters a = 68.4 A, b = 71.4 A, c = 74.8 A, alpha = 73.4, beta = 80.5, gamma = 72.3 A. Crystals grown in the presence of UDP-MurNAc-l-Ala-d-Glu alone belong to space group P2(1), with unit-cell parameters a = 71.1 A, b = 129.4 A, c = 74.6 A, beta = 106.3° Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.7 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.7
-
 Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
6.3.2.7 MuE
-
 Streptococcus pneumoniae
6.3.2.7 uridine 5'-diphospho-N-acetylmuramoyl L-alanyl-D-glutamate:lysine ligase
-
 Streptococcus pneumoniae