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Literature summary extracted from

  • Harjes, S.; Scheidig, A.; Bayer, P.
    Expression, purification and crystallization of human 3'-phosphoadenosine-5'-phosphosulfate synthetase 1 (2004), Acta Crystallogr. Sect. D, 60, 350-352.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.25 expressed as His-tag fusion protein in Escherichia coli BL21(DE3) Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.25 hanging drop vapor diffusion method Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.25 162000
-
 gel filtration Homo sapiens
2.7.1.25 716000
-
 2 * 716000, MALDI-TOF mass spectrometry, native mass by gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.25 ATP + adenosine 5'-phosphosulfate Homo sapiens synthesis of the important sulfate donor 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.25 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.25 recombinant protein using His-tag Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.25 ATP + adenosine 5'-phosphosulfate
-
 Homo sapiens ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?
2.7.1.25 ATP + adenosine 5'-phosphosulfate synthesis of the important sulfate donor 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate Homo sapiens ADP + 3'-phosphoadenosine 5'-phosphosulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.25 dimer 2 * 716000, MALDI-TOF mass spectrometry, native mass by gel filtration Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.1.25 More bifunctional enzyme with ATP sulfurylase activity and adenosine phosphosulfate kinase activity, catalyzes the formation of 3'-phosphoadenosine 5'-phosphosulfate from ATP and sulfate Homo sapiens
2.7.1.25 PAPS synthetase two isoforms, type 1 and 2 Homo sapiens