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Literature summary extracted from

  • Dwivedi, S.; Kruparani, S.P.; Sankaranarayanan, R.
    Cloning, expression, purification, crystallization and preliminary X-ray crystallographic investigations of a unique editing domain from archaebacteria (2004), Acta Crystallogr. Sect. D, 60, 1662-1664.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.3 DNA and amino acid sequence determination of residues 1-183, expression in Escherichia coli strain BL21(DE3) Pyrococcus abyssi

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.3 purified ultrafiltrated recombinant residues 1-183, hanging drop vapour diffusion method, protein solution, containing 50 mM Tris-HCl, pH 7.5, and 50 mM NaCl, drops of varying volumes against 0.75 ml reservoir solution, at room temperature or at 4°C, preliminary X-ray diffraction for structure determination at 1.95 A resolution Pyrococcus abyssi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.3 ATP + L-threonine + tRNAThr Pyrococcus abyssi
-
 AMP + diphosphate + L-threonyl-tRNAThr
-
 r

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.3 Pyrococcus abyssi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.3 recombinant residues 1-183 from Escherichia coli strain BL21(DE3) by ion exchange chromatography, ammonium sulfate fractionation and gel filtration to homogeneity Pyrococcus abyssi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.3 ATP + L-threonine + tRNAThr
-
 Pyrococcus abyssi AMP + diphosphate + L-threonyl-tRNAThr
-
 r
6.1.1.3 ATP + L-threonine + tRNAThr editing mechanism Pyrococcus abyssi AMP + diphosphate + L-threonyl-tRNAThr
-
 r

Synonyms

EC Number Synonyms Comment Organism
6.1.1.3 Threonyl-tRNA synthetase
-
 Pyrococcus abyssi
6.1.1.3 ThrRS
-
 Pyrococcus abyssi

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.3 AMP
-
 Pyrococcus abyssi
6.1.1.3 ATP
-
 Pyrococcus abyssi