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Literature summary extracted from

  • Crosby, J.
    Synthesis of optically active compounds: A large scale perspective (1991), Tetrahedron, 47, 4789-4846.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.14.12.10 synthesis production of (R,S)-1,2-dihydroxycatechol, which is used as a polymerisation monomer Pseudomonas putida
3.5.2.11 synthesis production of D-alpha-amino-beta-caprolactam and L-lysine as nutrient and food supplement Papiliotrema laurentii
4.3.1.24 synthesis production of L-phenylalanine, which is used in the manufacture of the artificial sweetener aspartame and in parenteral nutrition, it is also used as a building block for the synthesis of the macrolide antibiotic rutamycin B Rhodotorula mucilaginosa
4.3.1.24 synthesis production of L-phenylalanine, which is used in the manufacture of the artificial sweetener aspartame and in parenteral nutrition, it is also used as a building block for the synthesis of the macrolide antibiotic rutamycin B Rhodococcus rubra
5.1.1.15 synthesis production of DL-alpha-amino-beta-caprolactam as nutrient and food supplement Achromobacter obae

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.10 Pseudomonas putida
-
-
-
3.5.2.11 Papiliotrema laurentii
-
-
-
4.3.1.24 Rhodococcus rubra
-
Genex 1983
-
4.3.1.24 Rhodotorula mucilaginosa
-
Genex 1986
-
5.1.1.15 Achromobacter obae
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.3.1.24 Due to the instability of the enzyme towards oxygen, the biotransformation is performed under anaerobic, static conditions Rhodotorula mucilaginosa
4.3.1.24 Due to the instability of the enzyme towards oxygen, the biotransformation is performed under anaerobic, static conditions Rhodococcus rubra

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.10 benzene + O2 + NADH
-
Pseudomonas putida (R,S)-1,2-dihydro-1,2-dihydroxycatechol + NAD+
-
?
3.5.2.11 DL-alpha-amino-beta-caprolactam + H2O
-
Papiliotrema laurentii D-alpha-amino-beta-caprolactam + L-lysine
-
?
4.3.1.24 trans-cinnamic acid + NH3
-
Rhodotorula mucilaginosa L-phenylalanine
-
?
4.3.1.24 trans-cinnamic acid + NH3
-
Rhodococcus rubra L-phenylalanine
-
?
5.1.1.15 D-alpha-amino-beta-caprolactam
-
Achromobacter obae DL-alpha-amino-beta-caprolactam
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.11 40
-
-
Papiliotrema laurentii
4.3.1.24 25
-
-
Rhodotorula mucilaginosa
4.3.1.24 25
-
-
Rhodococcus rubra
5.1.1.15 40
-
-
Achromobacter obae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.24 10.6
-
-
Rhodotorula mucilaginosa
4.3.1.24 10.6
-
-
Rhodococcus rubra

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.2.11 8 9
-
Papiliotrema laurentii
5.1.1.15 8 9
-
Achromobacter obae