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Literature summary extracted from

  • Noshiro, M.; Ishida, H.; Hayashi, S.; Okuda, K.
    Assays for cholesterol 7alpha-hydroxylase and 12alpha-hydroxylase using high performance liquid chromatography (1985), Steroids, 45, 539-550.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.139 microsome
-
 Rattus norvegicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.139 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.139 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.139 0.000885
-
 liver microsome Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.139 7alpha-hydroxycholest-4-en-3-one + NADH + H+ + O2 reaction with NADH shows 15% of the activity with NADPH Rattus norvegicus 7alpha,12alpha-dihydroxycholest-4-en-3-one + NAD+ + H2O
-
 ?
1.14.14.139 7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2 NADPH is the preferred electron source Rattus norvegicus 7alpha,12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.139 HCO 12alpha-hydroxylase
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.139 NADH 15% of the activity with NADPH Rattus norvegicus
1.14.14.139 NADPH preferred electron source Rattus norvegicus