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Literature summary extracted from

  • Lee, S.G.; Lutz, S.; Benkovic, S.J.
    On the structural and functional modularity of glycinamide ribonucleotide formyltransferases (2003), Protein Sci., 12, 2206-2214.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.2 hybrid enzymes of human GART and PurN from Escherichia coli are expressed in Escherichia coli: PGX-B12 (PurN1-101/hGART102-202), GPX-M1 (hGART1-100/PurN101-212), GPX-M1 (hGART1129/PurN54-212), GPX-M24 (hGART1-54/PurN55-212), GPX-M36 (hGART1-89/PurN90-212), GPX-M55 (hGART1-56/PurN58-212), GPG-N11 (hGART1-56/PurN57-131/hGART132-202) Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.2 additional information hybrid enzymes of human GART and PurN from Escherichia coli are expressed in Escherichia coli: PGX-B12 (PurN1-101/hGART102-202),GPX-M1 (hGART1-100/PurN101-212), GPX-M1 (hGART1129/PurN54-212), GPX-M24 (hGART1-54/PurN55-212), GPX-M36 (hGART1-89/PurN90-212), GPX-M55 (hGART1-56/PurN58-212), GPG-N11 (hGART1-56/PurN57-131/hGART132-202) Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.2 0.99
-
 beta-glycinamide ribonucleotide chimeric enzyme GPG-N11 (hGART1-56/PurN57-131/hGART132-202) Homo sapiens
2.1.2.2 0.99
-
 10-formyl-5,8-dideazafolate chimeric enzyme GPG-N11 (hGART1-56/PurN57-131/hGART132-202) Homo sapiens
2.1.2.2 4
-
 beta-glycinamide ribonucleotide wild-type enzyme Homo sapiens
2.1.2.2 4
-
 10-formyl-5,8-dideazafolate wild-type enzyme Homo sapiens
2.1.2.2 4
-
 beta-glycinamide ribonucleotide chimeric enzyme GPX-M1 (hGART1-100/PurN101-212) Homo sapiens
2.1.2.2 4
-
 10-formyl-5,8-dideazafolate chimeric enzyme GPX-M1 (hGART1-100/PurN101-212) Homo sapiens
2.1.2.2 12
-
 beta-glycinamide ribonucleotide chimeric enzyme GPX-M55 (hGART1-56/PurN58-212) Homo sapiens
2.1.2.2 12
-
 10-formyl-5,8-dideazafolate chimeric enzyme GPX-M55 (hGART1-56/PurN58-212) Homo sapiens
2.1.2.2 21
-
 beta-glycinamide ribonucleotide chimeric enzyme GPX-M24 (hGART1-54/PurN55-212) Homo sapiens
2.1.2.2 21
-
 10-formyl-5,8-dideazafolate chimeric enzyme GPX-M24 (hGART1-54/PurN55-212) Homo sapiens
2.1.2.2 33
-
 beta-glycinamide ribonucleotide chimeric enzyme PGX-B12 (PurN1-101/hGART102-202) Homo sapiens
2.1.2.2 33
-
 10-formyl-5,8-dideazafolate chimeric enzyme PGX-B12 (PurN1-101/hGART102-202) Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.2
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.2 10-formyl-5,8-dideazafolate + beta-glycinamide ribonucleotide
-
 Homo sapiens 5,8-dideazafolate + N-formyl beta-glycinamide ribonucleotide
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.2 0.0019
-
 10-formyl-5,8-dideazafolate chimeric enzyme GPX-M1 (hGART1-100/PurN101-212) Homo sapiens
2.1.2.2 0.0034
-
 beta-glycinamide ribonucleotide wild-type enzyme Homo sapiens
2.1.2.2 0.0059
-
 10-formyl-5,8-dideazafolate wild-type enzyme Homo sapiens
2.1.2.2 0.018
-
 10-formyl-5,8-dideazafolate chimeric enzyme GPX-M24 (hGART1-54/PurN55-212) Homo sapiens
2.1.2.2 0.0262
-
 beta-glycinamide ribonucleotide chimeric enzyme PGX-B12 (PurN1-101/hGART102-202) Homo sapiens
2.1.2.2 0.031
-
 10-formyl-5,8-dideazafolate chimeric enzyme GPX-M55 (hGART1-56/PurN58-212) Homo sapiens
2.1.2.2 0.043
-
 10-formyl-5,8-dideazafolate chimeric enzyme GPG-N11 (hGART1-56/PurN57-131/hGART132-202) Homo sapiens
2.1.2.2 0.0662
-
 beta-glycinamide ribonucleotide chimeric enzyme GPX-M1 (hGART1-100/PurN101-212) Homo sapiens
2.1.2.2 0.102
-
 10-formyl-5,8-dideazafolate chimeric enzyme PGX-B12 (PurN1-101/hGART102-202) Homo sapiens
2.1.2.2 0.262
-
 beta-glycinamide ribonucleotide chimeric enzyme GPX-M24 (hGART1-54/PurN55-212) Homo sapiens
2.1.2.2 0.293
-
 beta-glycinamide ribonucleotide chimeric enzyme GPG-N11 (hGART1-56/PurN57-131/hGART132-202) Homo sapiens
2.1.2.2 0.63
-
 beta-glycinamide ribonucleotide chimeric enzyme GPX-M55 (hGART1-56/PurN58-212) Homo sapiens