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Literature summary extracted from

  • Pinotsis, N.; Leonidas, D.D.; Chrysina, E.D.; Oikonomakos, N.G.; Mavridis, I.M.
    The binding of beta- and gamma-cyclodextrins to glycogen phosphorylase b: kinetic and crystallographic studies (2003), Protein Sci., 12, 1914-1924.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.1.1 additional information the enzyme is a target for development of inhibitors to prevent unwanted hepatic glycogenolysis under high glucose conditions in treatment of diabetes type 2 in humans Oryctolagus cuniculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.1 native enzyme crystals are soaked in 150 mM gamma-cyclodextrin, or 15 mM beta-cyclodextrin, respectively, and 70 mM maltopentaose or maltoheptaose in fesh solutions of mother liquor containing 10 mM MES, pH 6.7, 0.1 mM EDTA, at least 2 h at room temperature, X-ray diffraction structure determination and analysis of enzyme-inhibitor complexes at 2.3 A and 1.94 A resolution, respectively Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.1 alpha-cyclodextrin mixed-type competitive inhibition, inhibitor is not bound into the enzyme crystal Oryctolagus cuniculus
2.4.1.1 beta-cyclodextrin mixed-type competitive inhibition, the inhibitor can be accomodated in the glycogen storage site of T-state enzyme, subsite specificity Oryctolagus cuniculus
2.4.1.1 gamma-cyclodextrin mixed-type competitive inhibition, the inhibitor can be accomodated in the glycogen storage site of T-state enzyme, subsite specificity Oryctolagus cuniculus
2.4.1.1 additional information binding and inhibition mechanism, no inhibition by (2,3,6-tri-O-methyl)-gamma-cyclodextrin Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.1 Oryctolagus cuniculus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.1 [(1->4)-alpha-D-glucosyl]n + phosphate = [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate determination and analysis of rgulatory binding sites Oryctolagus cuniculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.1 muscle
-
 Oryctolagus cuniculus
-

Synonyms

EC Number Synonyms Comment Organism
2.4.1.1 glycogen phosphorylase
-
 Oryctolagus cuniculus
2.4.1.1 GP
-
 Oryctolagus cuniculus
2.4.1.1 GPb
-
 Oryctolagus cuniculus
2.4.1.1 phosphorylase b
-
 Oryctolagus cuniculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.1 7.4
-
 gamma-cyclodextrin with respect to glycogen Oryctolagus cuniculus
2.4.1.1 14.1
-
 beta-cyclodextrin with respect to glycogen Oryctolagus cuniculus
2.4.1.1 47.1
-
 alpha-cyclodextrin with respect to glycogen Oryctolagus cuniculus