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Literature summary extracted from

  • Karlsson, J.; Nilsson, T.
    The C subunit of Ideonella dechloratans chlorate reductase: expression, purification, refolding, and heme reconstitution (2005), Protein Expr. Purif., 41, 306-312.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.97.1.1 C-subunit expressed as GST-fusion protein in Escherichia coli BL21 Ideonella dechloratans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.97.1.1 periplasm
-
 Ideonella dechloratans
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.97.1.1 Iron non-heme iron Ideonella dechloratans
1.97.1.1 Molybdenum bound to enzyme Ideonella dechloratans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.97.1.1 chlorate + reduced electron acceptor Ideonella dechloratans important enzyme in bacterial treatment of bleach effluents in pulp and paper industry chlorite + H2O + oxidized electron acceptor
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.97.1.1 Ideonella dechloratans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.97.1.1 inclusion bodies from Escherichia coli and native enzyme Ideonella dechloratans

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.97.1.1 refolding Ideonella dechloratans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.97.1.1 bromate + reduced electron acceptor
-
 Ideonella dechloratans bromite + H2O + oxidized electron acceptor
-
 ?
1.97.1.1 chlorate + reduced electron acceptor
-
 Ideonella dechloratans chlorite + H2O + oxidized electron acceptor
-
 ?
1.97.1.1 chlorate + reduced electron acceptor important enzyme in bacterial treatment of bleach effluents in pulp and paper industry Ideonella dechloratans chlorite + H2O + oxidized electron acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.97.1.1 trimer
-
 Ideonella dechloratans

Cofactor

EC Number Cofactor Comment Organism Structure
1.97.1.1 heme b
-
 Ideonella dechloratans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.97.1.1 Ideonella dechloratans calculated pI of expressed GST-fusion protein
-
 8.6