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Literature summary extracted from

  • Fang, J.; Ewald, D.
    Expression cloned cDNA for 10-deacetylbaccatin III-10-O-acetyltransferase in Escherichia coli: a comparative study of three fusion systems (2004), Protein Expr. Purif., 35, 17-24.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.167 overexpression in Escherichia coli. Three kinds of fusion systems with isopropyl-beta-D-1-thiogalactopyranoside inducible promoters are used. The fusion partners are glutathione S-transferase tag, hexahistidine fusion tag, and a biotinylated peptide tag Taxus baccata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.167 0.0085
-
 acetyl-CoA recombinant enzyme with a hexahistidine fusion tag Taxus baccata
2.3.1.167 0.011
-
 10-deacetylbaccatin III recombinant enzyme with a hexahistidine fusion tag Taxus baccata

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.167 Taxus baccata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.167
-
 Taxus baccata

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.167 6.1
-
 recombinant enzyme with biotinylated peptide tag Taxus baccata
2.3.1.167 16.1
-
 recombinant glutathione S-transferase-fusion protein Taxus baccata
2.3.1.167 24.4
-
 recombinant enzyme with a hexahistidine fusion tag Taxus baccata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.167 acetyl-CoA + 10-deacetylbaccatin III
-
 Taxus baccata CoA + baccatin III
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.167 10-DABT
-
 Taxus baccata
2.3.1.167 10-deacetylbaccatin III-10-O-acetyltransferase
-
 Taxus baccata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.167 30
-
-
 Taxus baccata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.167 7.4
-
 recombinant enzymes Taxus baccata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.167 6 8 half-maximal aactivity of recombinant enzyme at pH 6.0 and 8.0 Taxus baccata