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Literature summary extracted from

  • Booth, R.E.; Misquitta, S.A.; Bateman, R.C., Jr.
    Human pituitary glutaminyl cyclase: expression in insect cells and dye affinity purification (2003), Protein Expr. Purif., 32, 141-146.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.5 expression in Drosophila S2 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.5 N49A mutant enzyme is not glycosylated, kinetic properties are indistinguishable from unmutated enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.5 0.7
-
 Gln-Gln
-
 Homo sapiens
2.3.2.5 1.4
-
 Gln-NH2
-
 Homo sapiens
2.3.2.5 6.7
-
 Gln-tert-butyl ester
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.5 37000
-
 x * 37000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.5 Bos taurus
-
-
-
2.3.2.5 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.2.5 glycoprotein
-
 Bos taurus
2.3.2.5 glycoprotein N-glycan sugar moiety is bound to Asn49 in the recombinant enzyme. Presence or absence of glycan has no apparent effect on structure, stability, or enzymatic activity of the enzyme Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.5
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.5 pituitary gland
-
 Homo sapiens
-
2.3.2.5 pituitary gland
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.5 Gln-Gln
-
 Homo sapiens pyroglutamyl-Gln + NH3
-
 ?
2.3.2.5 Gln-tert-butyl ester
-
 Homo sapiens pyroglutamyl-tert-butyl ester + NH3
-
 ?
2.3.2.5 L-Gln-NH2
-
 Homo sapiens L-pyroglutamyl amide + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.5 ? x * 37000, SDS-PAGE Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.2.5 10
-
 Gln-Gln
-
 Homo sapiens
2.3.2.5 13
-
 Gln-NH2
-
 Homo sapiens
2.3.2.5 16
-
 Gln-tert-butyl ester
-
 Homo sapiens