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Literature summary extracted from

  • Coffa, G.; Brash, A.R.
    A single active site residue directs oxygenation stereospecificity in lipoxygenases: Stereocontrol is linked to the position of oxygenation (2004), Proc. Natl. Acad. Sci. USA, 101, 15579-15584.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.31 expression in Escherichia coli Homo sapiens
1.13.11.33 expression in Escherichia coli Homo sapiens
1.13.11.40 expression in HeLa cells Plexaura homomalla

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.31 G441A the mutant enzyme G441A converts arachidonic acid to (8S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid and (12R)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid in a 1.4:1 ratio, wild-type enzyme converts arachidonate only to (12R)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid Homo sapiens
1.13.11.31 G441S the mutant enzyme G441A converts arachidonic acid to 8S-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid (major product) and (12R)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid (minor product), wild-type enzyme converts arachidonate only to (12R)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid Homo sapiens
1.13.11.31 G441T inactive mutant enzyme Homo sapiens
1.13.11.31 G441V inactive mutant enzyme Homo sapiens
1.13.11.33 A416T inactive mutant protein Homo sapiens
1.13.11.33 A416V inactive mutant protein Homo sapiens
1.13.11.33 A417G the mutant enzyme converts arachidonic acid to (11R)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid and (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid in a 1.5:1 ratio. The wild.type enzyme converts arachidonic acid exlusively (15S)-Hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid. Turnover number for arachidonic acid is 4.6fold lower than that of the wild-type enzyme, no significant change in Km-value Homo sapiens
1.13.11.40 A417G converts arachidonic acid mainly to 12-hydroxyeicosatetraenoic acid, mutant retains 38% of catalytic efficiency Plexaura homomalla
1.13.11.40 A417S same oxygenase specificity and similar catalytic activity to wild-type 8S-LOX Plexaura homomalla

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.40 0.008
-
 arachidonate
-
 Plexaura homomalla

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.40 arachidonate + O2 Plexaura homomalla
-
 (5Z,9E,11Z,14Z)-(8S)-8-hydroperoxyeicosa-5,9,11,14-tetraenoate
-
 ?
1.13.11.40 arachidonate + O2 Plexaura homomalla
-
 (5Z,9E,11Z,14Z)-(8S)-8-hydroperoxyicosa-5,9,11,14-tetraenoate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.31 Homo sapiens
-
-
-
1.13.11.33 Homo sapiens
-
-
-
1.13.11.40 Plexaura homomalla
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.31 arachidonic acid + O2
-
 Homo sapiens (12R)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoic acid the mutant enzyme G441A converts arachidonic acid to 8S-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid and 12R-hydroperoxy-5Z,8Z,11Z,13E-eicosatetraenoic acid in a 1.4:1 ratio ?
1.13.11.33 arachidonic acid + O2
-
 Homo sapiens (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid the mutant enzyme A416G converts arachidonic acid to (11R)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid and (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid in a 1.5:1 ratio ?
1.13.11.40 arachidonate + O2
-
 Plexaura homomalla (5Z,9E,11Z,14Z)-(8S)-8-hydroperoxyeicosa-5,9,11,14-tetraenoate
-
 ?
1.13.11.40 arachidonate + O2
-
 Plexaura homomalla (5Z,9E,11Z,14Z)-(8S)-8-hydroperoxyicosa-5,9,11,14-tetraenoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.31 12R-LOX-2
-
 Homo sapiens
1.13.11.33 15-LOX-2
-
 Homo sapiens
1.13.11.40 8S-LOX
-
 Plexaura homomalla

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.31 3
-
 arachidonate mutant enzyme A416G Homo sapiens
1.13.11.31 14
-
 arachidonate wild-type enzyme Homo sapiens
1.13.11.33 3
-
 arachidonate mutant enzyme A416G Homo sapiens
1.13.11.33 14
-
 arachidonate wild-type enzyme Homo sapiens
1.13.11.40 0.3
-
 arachidonate
-
 Plexaura homomalla