BRENDA - Enzyme Database

Switching desaturase enzyme specificity by alternate subcellular targeting

Heilmann, I.; Pidkowich, M.S.; Girke, T.; Shanklin, J.; Proc. Natl. Acad. Sci. USA 101, 10266-10271 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.19.42
coexpressed with cucumber monogalactosyldiacylglycerol synthase in Saccharomyces cerevisiae OLE1 strain
Arabidopsis thaliana
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.14.19.1
additional information
fusion protein of enzyme isoform ADS1 or ADS2 with plastid targeting sequence of palmitic acid DELTA7 desaturase targets enzymes to plastid and shifts regiospecificity of enzymes from DELTA9 desaturase to DELTA7 desaturase
Arabidopsis thaliana
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.1
cytoplasm
-
Arabidopsis thaliana
5737
-
1.14.19.42
plastid
-
Arabidopsis thaliana
9536
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.42
1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Arabidopsis thaliana
-
1-acyl-2-[(7Z)-hexadec-7-enoyl]-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.19.1
Arabidopsis thaliana
-
isoform ADS1, ADS2
-
1.14.19.42
Arabidopsis thaliana
Q949X0
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.42
1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
660359
Arabidopsis thaliana
1-acyl-2-[(7Z)-hexadec-7-enoyl]-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.19.42
coexpressed with cucumber monogalactosyldiacylglycerol synthase in Saccharomyces cerevisiae OLE1 strain
Arabidopsis thaliana
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.14.19.1
additional information
fusion protein of enzyme isoform ADS1 or ADS2 with plastid targeting sequence of palmitic acid DELTA7 desaturase targets enzymes to plastid and shifts regiospecificity of enzymes from DELTA9 desaturase to DELTA7 desaturase
Arabidopsis thaliana
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.1
cytoplasm
-
Arabidopsis thaliana
5737
-
1.14.19.42
plastid
-
Arabidopsis thaliana
9536
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.42
1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Arabidopsis thaliana
-
1-acyl-2-[(7Z)-hexadec-7-enoyl]-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.42
1-acyl-2-palmitoyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
660359
Arabidopsis thaliana
1-acyl-2-[(7Z)-hexadec-7-enoyl]-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?