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Literature summary extracted from
- Purification and partial characterization of manganese peroxidase from immobilized Phanerochaete chrysosporium (2004), Proc. Biochem., 39, 2061-2068.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.11.1.13 | complete loss of activity after 1 h in 2 mM KCN | Phanerodontia chrysosporium |
| 1.11.1.13 | no inactivation after 5 h in 50 mM Na-tartrate buffer, pH 4.5, with 2 mM nitrilotriacetate, 0.5 mM iodoacetamide or 2 mM PMSF | Phanerodontia chrysosporium |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.13 | acetate | 1 mM, 58% inhibition | Phanerodontia chrysosporium |  |
| 1.11.1.13 | ascorbic acid | 2 mM, complete inhibition | Phanerodontia chrysosporium | |
| 1.11.1.13 | beta-mercaptoethanol | 2 mM, complete inhibition | Phanerodontia chrysosporium | |
| 1.11.1.13 | N-bromosuccinimide | - |
Phanerodontia chrysosporium | |
| 1.11.1.13 | N-ethyl-5-phenylisoxazolium 3'-sulfonate | 1 mM, 28% inhibition | Phanerodontia chrysosporium | |
| 1.11.1.13 | NaN3 | 2 mM, complete inhibition | Phanerodontia chrysosporium | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.13 | 0.0286 | - |
2,6-dimethoxyphenol | 30°C, pH 4.5 | Phanerodontia chrysosporium | |
| 1.11.1.13 | 0.0714 | - |
H2O2 | 30°C, pH 4.5 | Phanerodontia chrysosporium | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.13 | Mn2+ | the proposed role of Mn2+ chelators in the enzyme mechanism is to release Mn3+ from the enzyme | Phanerodontia chrysosporium | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.13 | 45000 | - |
gel filtration | Phanerodontia chrysosporium |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.11.1.13 | Phanerodontia chrysosporium | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.11.1.13 | - |
Phanerodontia chrysosporium |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.13 | 484.8 | - |
- |
Phanerodontia chrysosporium |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.11.1.13 | -20°C, 10% decrease of activity after 3 months | Phanerodontia chrysosporium |
| 1.11.1.13 | 4°C, pH 4.5, purified enzyme in 50 mM Na-tartrate buffer is stable for 1 week | Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.13 | 2,6-dimethoxyphenol + H2O2 | - |
Phanerodontia chrysosporium | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.11.1.13 | monomer | 1 * 45000, SDS-PAGE | Phanerodontia chrysosporium |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.13 | 30 | - |
- |
Phanerodontia chrysosporium |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.13 | 25 | 45 | 25°C: about 80% of maximal activity, 45°C: about 60% of maximal activity | Phanerodontia chrysosporium |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.13 | 35 | - |
pH 4.5, 1 h, stable up to | Phanerodontia chrysosporium |
| 1.11.1.13 | 45 | - |
pH 4.5, 1 h, about 40% loss of activity | Phanerodontia chrysosporium |
| 1.11.1.13 | 47 | - |
pH 4.5, 1 h, about 60% loss of activity | Phanerodontia chrysosporium |
| 1.11.1.13 | 65 | - |
pH 4.5, 1 h, complete loss of activity | Phanerodontia chrysosporium |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.13 | 4.5 | - |
- |
Phanerodontia chrysosporium |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.13 | 3.5 | 6 | pH 3.5: about 30% of maximal activity, pH 4.0: about 70% of maximal activity, pH 6.0: about 45% of maximal activity | Phanerodontia chrysosporium |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.13 | 2.5 | - |
25°C, 2 h, about 50% loss of activity | Phanerodontia chrysosporium |
| 1.11.1.13 | 3 | - |
25°C, 2 h, about 40% loss of activity | Phanerodontia chrysosporium |
| 1.11.1.13 | 4.5 | 6 | 25°C, 2 h, stable | Phanerodontia chrysosporium |
| 1.11.1.13 | 6.5 | - |
25°C, 2 h, about 20% loss of activity | Phanerodontia chrysosporium |
| 1.11.1.13 | 7 | - |
25°C, 2 h, about 40% loss of activity | Phanerodontia chrysosporium |
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