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Literature summary extracted from

  • Milkowski, C.; Baumert, A.; Strack, D.
    Cloning and heterologous expression of a rape cDNA encoding UDP-glucose:sinapate glucosyltransferase (2000), Planta, 211, 883-886.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.120 construction of a cDNA library, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain M15 as soluble protein Brassica napus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.120 55970
-
 x * 55970, amino acid sequence calculation, mass spectrometry Brassica napus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.120 UDP-alpha-D-glucose + sinapate Brassica napus
-
 UDP + 1-O-sinapoyl-beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.120 Brassica napus Q9FYU7 SGT1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.120 cotyledon from 3-days-old seedlings Brassica napus
-
2.4.1.120 seedling
-
 Brassica napus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.120 0.018
-
 recombinant enzyme, substrate sinapate Brassica napus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.120 additional information recombinant enzyme shows a broad substrate specificity, indole-3-acetate, 4-hydroxybenzoate, and salicylate are no substrates Brassica napus ?
-
 ?
2.4.1.120 UDP-alpha-D-glucose + 4-coumarate 13% activity compared to sinapate Brassica napus UDP + 1-O-(4-coumaroyl)-beta-D-glucose
-
 ?
2.4.1.120 UDP-alpha-D-glucose + cinnamate 20% activity compared to sinapate Brassica napus UDP + 1-O-cinnamoyl-beta-D-glucose
-
 ?
2.4.1.120 UDP-alpha-D-glucose + ferulate 17% activity compared to sinapate Brassica napus UDP + 1-O-feruloyl-beta-D-glucose
-
 ?
2.4.1.120 UDP-alpha-D-glucose + sinapate
-
 Brassica napus UDP + 1-O-sinapoyl-beta-D-glucose
-
 ?
2.4.1.120 UDP-alpha-D-glucose + sinapate best substrate Brassica napus UDP + 1-O-sinapoyl-beta-D-glucose
-
 ?
2.4.1.120 UDP-glucose + caffeate 10% activity compared to sinapate Brassica napus UDP + 1-caffeoyl-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.120 ? x * 55970, amino acid sequence calculation, mass spectrometry Brassica napus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.120 SGT
-
 Brassica napus
2.4.1.120 UDP-glucose:sinapate glucosyltransferase
-
 Brassica napus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.120 30
-
 assay at Brassica napus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.120 6
-
 assay at Brassica napus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.120 Brassica napus sequence calculation
-
 6.1