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Literature summary extracted from

  • Havir, E.A.; Tausta, S.L.; Peterson, R.B.
    Purification and properties of violaxanthin de-epoxidase from spinach (1997), Plant Sci., 123, 57-66.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.23.5.1 Cys 2.7 mM, 50% inhibition Spinacia oleracea
1.23.5.1 dithiothreitol 0.055 mM, 50% inhibition. The rate of conversion of violaxanthin to antheraxanthin is relatively unchanged whereas the conversion of antheraxanthin to zeaxanthin is 50-80% inhibited Spinacia oleracea
1.23.5.1 mercaptoethanol 0.68 mM, 50% inhibition Spinacia oleracea
1.23.5.1 o-phenanthroline 0.025 mM, 50% inhibition. The rate of conversion of violaxanthin to antheraxanthin is relatively unchanged whereas the conversion of antheraxanthin to zeaxanthin is 50-80% inhibited Spinacia oleracea
1.23.5.1 zeaxanthin product inhibition Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.23.5.1 0.005
-
 violaxanthin
-
 Spinacia oleracea
1.23.5.1 0.0053
-
 antheraxanthin
-
 Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.23.5.1 thylakoid
-
 Spinacia oleracea 9579
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.23.5.1 43300
-
 1 * 43300, SDS-PAGE Spinacia oleracea
1.23.5.1 45800
-
 gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.23.5.1 antheraxanthin + ascorbate Spinacia oleracea
-
 zeaxanthin + dehydroascorbate + H2O
-
 ?
1.23.5.1 violaxanthin + ascorbate Spinacia oleracea
-
 antheraxanthin + dehydroascorbate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.23.5.1 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.23.5.1
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.23.5.1 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.23.5.1 19
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.23.5.1 antheraxanthin + ascorbate
-
 Spinacia oleracea zeaxanthin + dehydroascorbate + H2O
-
 ?
1.23.5.1 violaxanthin + ascorbate
-
 Spinacia oleracea antheraxanthin + dehydroascorbate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.23.5.1 monomer 1 * 43300, SDS-PAGE Spinacia oleracea

Synonyms

EC Number Synonyms Comment Organism
1.23.5.1 VDE
-
 Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.23.5.1 5.2
-
-
 Spinacia oleracea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.23.5.1 Spinacia oleracea isoelectric focusing
-
 4.95