Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sauer, P.; Frebortova, J.; Sebela, M.; Galuszka, P.; Jacobsen, S.; Pec, P.; Frebort, I.
    Xanthine dehydrogenase of pea seedlings: a member of the plant molybdenum oxidoreductase family (2002), Plant Physiol. Biochem., 40, 393-400.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.4 adenine 0.1 mM, 21% residual activity Pisum sativum
1.17.1.4 allopurinol 0.1 mM, complete inhibition Pisum sativum
1.17.1.4 N6-furfuryladenine 0.1 mM, 59% residual activity Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.4 0.017
-
 xanthine pH 8.5 Pisum sativum
1.17.1.4 0.018
-
 hypoxanthine pH 8.5 Pisum sativum
1.17.1.4 0.025
-
 NAD+ pH 8.5 Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.4 150000
-
 2 * 150000, SDS-PAGE Pisum sativum
1.17.1.4 300000
-
 gel filtration Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.4 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.4
-
 Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.4 seedling etiolated Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17.1.4 1.17
-
 pH 8.5 Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.4 hypoxanthine + NAD+ + H2O 44% of the activity with xanthine Pisum sativum xanthine + NADH + H+
-
 ?
1.17.1.4 indole-3-acetaldehyde + NAD+ + H2O and similar aldehydes, 2-3% of the activity with xanthine Pisum sativum indole-3-acetate + NADH + H+
-
 ?
1.17.1.4 xanthine + NAD+ + H2O
-
 Pisum sativum urate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.4 dimer 2 * 150000, SDS-PAGE Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.4 8.5
-
-
 Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.4 NAD+
-
 Pisum sativum
1.17.1.4 NADH
-
 Pisum sativum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.17.1.4 Pisum sativum isoelectric focusing
-
 5.3