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Literature summary extracted from

  • Thorsoe, K.S.; Bak, S.; Olsen, C.E.; Imberty, A.; Breton, C.; Moller, B.L.
    Determination of catalytic key amino acids and UDP sugar donor specificity of the cyanohydrin glycosyltransferase UGT85B1 from Sorghum bicolor. Molecular modeling substantiated by site-specific mutagenesis and biochemical analyses (2005), Plant Physiol., 139, 664-673.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.85 mutant enzymes, expression in Escherichia coli Sorghum bicolor

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.85 E410A glucosylation of mandelonitrile is 225fold reduced compared to wild-type enzyme Sorghum bicolor
2.4.1.85 R201A glucosylation of mandelonitrile is 20fold reduced compared to wild-type enzyme Sorghum bicolor
2.4.1.85 S391A glucosylation of mandelonitrile is 185fold reduced compared to wild-type enzyme Sorghum bicolor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.85 0.8
-
 UDP-glucose pH 7.5, 30°C, cosubstrate: mandelonitrile Sorghum bicolor

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.85 Sorghum bicolor Q9SBL1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.85 UDP-glucose + mandelonitrile specific for UDP-glucose Sorghum bicolor UDP + mandelonitrile beta-D-glucoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.85 cyanohydrin glycosyltransferase
-
 Sorghum bicolor
2.4.1.85 UGT85B1
-
 Sorghum bicolor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.85 8.9
-
 UDP-glucose pH 7.5, 30°C, cosubstrate: mandelonitrile Sorghum bicolor