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Literature summary extracted from

  • Zhou, R.; Yu, M.; Pharis, R.P.
    16,17-dihydro gibberellin A5 competitively inhibits a recombinant Arabidopsis GA 3beta-hydroxylase encoded by the GA4 gene (2004), Plant Physiol., 135, 1000-1007.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.15 expression as a GST-fusion protein in Escherichia coli Arabidopsis sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.11.15 endo-16,17-dihydro gibberellin A5 competitive inhibitor of 3beta-hydroxylation of gibberellin A20 Arabidopsis sp.
1.14.11.15 exo-16,17-dihydro gibberellin A20 competitive inhibitor of 3beta-hydroxylation of gibberellin A20 Arabidopsis sp.
1.14.11.15 exo-16,17-dihydro gibberellin A5 competitive inhibitor of 3beta-hydroxylation of gibberellin A20 Arabidopsis sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.15 0.00125
-
 gibberellin a9
-
 Arabidopsis sp.
1.14.11.15 0.01
-
 gibberellin a20
-
 Arabidopsis sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.15 Arabidopsis sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.15 gibberellin a20 + 2-oxoglutarate + O2 GST-fusion protein Arabidopsis sp. gibberellin a1 + succinate + CO2
-
 ?
1.14.11.15 gibberellin A5 + 2-oxoglutarate + O2
-
 Arabidopsis sp. gibberellin A6 + succinate + CO2
-
 ?
1.14.11.15 gibberellin a9 + 2-oxoglutarate + O2
-
 Arabidopsis sp. gibberellin a4 + succinate + CO2
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.15 7.5
-
-
 Arabidopsis sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.11.15 6 9 activity is reduced appreciably below pH 6 and above pH 9 Arabidopsis sp.