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Literature summary extracted from

  • Domergue, F.; Spiekermann, P.; Lerchl, J.; Beckmann, C.; Kilian, O.; Kroth, P.G.; Boland, W.; Zahringer, U.; Heinz, E.
    New insight into Phaeodactylum tricornutum fatty acid metabolism. Cloning and functional characterization of plastidial and microsomal DELTA12-fatty acid desaturases (2003), Plant Physiol., 131, 1648-1660.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.6 heterologous expression in yeast Saccharomyces cerevisiae and Synechococcus Phaeodactylum tricornutum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.6 microsome PtFAD2 Phaeodactylum tricornutum
-
-
1.14.19.6 plastid PtFAD6 Phaeodactylum tricornutum 9536
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.6 cis-9-octadecenoic acid + reduced acceptor + O2 Phaeodactylum tricornutum PtFAD2 is involved in the biosynthesis of eicosapentaenoic acid cis,cis-9,12-octadecadienoic acid + acceptor + 2 H2O
-
 ?
1.14.19.6 additional information Phaeodactylum tricornutum PtFAD6 is involved in the biosynthesis of hexadecantrienic acid ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.6 Phaeodactylum tricornutum
-
 PtFAD2
-
1.14.19.6 Phaeodactylum tricornutum
-
 PtFAD6
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.6 cis-7-hexadecenoic acid + reduced acceptor + O2 5.2% desaturation Phaeodactylum tricornutum cis,cis-7,10-hexadecadienoic acid + acceptor + H2O
-
 ?
1.14.19.6 cis-9-heptadecenoic acid + reduced acceptor + O2 22.3% desaturation Phaeodactylum tricornutum cis,cis-9,12-heptadecadienoic acid + acceptor + H2O
-
 ?
1.14.19.6 cis-9-hexadecenoic acid + reduced acceptor + O2 14.7% desaturation Phaeodactylum tricornutum cis,cis-9,12-hexadecadienoic acid + acceptor + H2O
-
 ?
1.14.19.6 cis-9-hexadecenoic acid + reduced acceptor + O2 70.3% desaturation Phaeodactylum tricornutum cis,cis-9,12-hexadecadienoic acid + acceptor + H2O
-
 ?
1.14.19.6 cis-9-icosenoic acid + reduced acceptor + O2 4.1% desaturation Phaeodactylum tricornutum cis,cis-11,14-icosadienoic acid + acceptor + H2O
-
 ?
1.14.19.6 cis-9-octadecenoic acid + reduced acceptor + O2 PtFAD2 is involved in the biosynthesis of eicosapentaenoic acid Phaeodactylum tricornutum cis,cis-9,12-octadecadienoic acid + acceptor + 2 H2O
-
 ?
1.14.19.6 cis-9-octadecenoic acid + reduced acceptor + O2 20.6% desaturation Phaeodactylum tricornutum cis,cis-9,12-octadecadienoic acid + acceptor + 2 H2O
-
 ?
1.14.19.6 cis-9-octadecenoic acid + reduced acceptor + O2 cis-9-octadecenoic acid is the most efficient substrate for PtFAD2, 50.3% desaturation Phaeodactylum tricornutum cis,cis-9,12-octadecadienoic acid + acceptor + 2 H2O
-
 ?
1.14.19.6 additional information PtFAD6 is involved in the biosynthesis of hexadecantrienic acid Phaeodactylum tricornutum ?
-
 ?
1.14.19.6 additional information no activity with cis-13-docosenoic acid Phaeodactylum tricornutum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.6 DELTA12(omega6)-desaturase
-
 Phaeodactylum tricornutum
1.14.19.6 DELTA12-fatty acyl-CoA desaturase
-
 Phaeodactylum tricornutum
1.14.19.6 oleoyl coenzyme A desaturase
-
 Phaeodactylum tricornutum
1.14.19.6 oleoyl-CoA DELTA12 desaturase
-
 Phaeodactylum tricornutum
1.14.19.6 PtFAD2
-
 Phaeodactylum tricornutum
1.14.19.6 PtFAD6
-
 Phaeodactylum tricornutum