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Literature summary extracted from

  • Leonard, R.; Kolarich, D.; Paschinger, K.; Altmann, F.; Wilson, I.B.H.
    A genetic and structural analysis of the N-glycosylation capabilities of rice and other monocotyledons (2004), Plant Mol. Biol., 55, 631-644.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.38 DNA and amino acid sequence determination and analysis, gene is located on chromosome 8, functional expression of the enzyme in Pichia pastoris as soluble protein, secretion to the cell culture medium Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.38 Co2+
-
 Oryza sativa
2.4.2.38 Cu2+
-
 Oryza sativa
2.4.2.38 EDTA
-
 Oryza sativa
2.4.2.38 Zn2+
-
 Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.38 0.055
-
 dansylated Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,6)-(Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,3))-Manbeta-(1,4)-GlcNAcbeta-(1,4)-GlcNAc recombinant soluble enzyme, pH 6.0, 16°C Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.38 Golgi apparatus
-
 Oryza sativa 5794
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.38 Ca2+ activates, enzyme requires divalent cations for full activity Oryza sativa
2.4.2.38 Fe2+ activates, enzyme requires divalent cations for full activity Oryza sativa
2.4.2.38 Mg2+ activates, enzyme requires divalent cations for full activity Oryza sativa
2.4.2.38 Mn2+ activates, enzyme requires divalent cations for full activity Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.38 additional information Oryza sativa enzyme is involved in N-glycan biosynthesis, quantification and structure analysis of N-glycans in rice leaves ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.38 Oryza sativa Q6ZFH6
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.38 leaf
-
 Oryza sativa
-

Storage Stability

EC Number Storage Stability Organism
2.4.2.38 4°C, purified recombinant enzyme, 3 weeks stable without loss of activity Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.38 additional information enzyme is involved in N-glycan biosynthesis, quantification and structure analysis of N-glycans in rice leaves Oryza sativa ?
-
 ?
2.4.2.38 UDP-xylose + dansylated Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,6)-(Galbeta-(1,4)-GlcNAcbeta-(1,2)-Manalpha-(1,3))-Manbeta-(1,4)-GlcNAcbeta-(1,4)-GlcNAc i.e. dansylated GnGn-peptide Oryza sativa UDP + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.38 beta1,2-xylosyltransferase
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.38 16
-
-
 Oryza sativa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.2.38 additional information
-
 low activity at 30°C Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.38 6.7
-
-
 Oryza sativa