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Literature summary extracted from

  • Verhaest, M.; Ende, W.V.; Roy, K.L.; De Ranter, C.J.; Laere, A.V.; Rabijns, A.
    X-ray diffraction structure of a plant glycosyl hydrolase family 32 protein: fructan 1-exohydrolase IIa of Cichorium intybus (2005), Plant J., 41, 400-411.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.80 heterologous expression in Pichia pastoris Cichorium intybus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.80 2.35 A resolution, hanging drop vapour diffusion, structure determination by single anomalous dispersion method Cichorium intybus
3.2.1.153 hanging drop vapour diffusion method of recombinant enzyme heterologously expressed in Pichia pastoris. The structure of the isoenzyme 1-FEH IIa is determined at a resolution of 2.35 A. The structure consists of an N-terminal fivefold beta-propeller domain Cichorium intybus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.153 additional information Cichorium intybus the enzyme is involved in fructan degradation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.80 Cichorium intybus Q93X60 isoenzyme 1-FEH IIa
-
3.2.1.153 Cichorium intybus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.80 glycoprotein
-
 Cichorium intybus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.80
-
 Cichorium intybus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.80 inulin + H2O
-
 Cichorium intybus beta-D-fructose + ?
-
 ?
3.2.1.80 additional information no activity towards sucrose Cichorium intybus ?
-
 ?
3.2.1.153 inulin + H2O
-
 Cichorium intybus fructose + ?
-
 ?
3.2.1.153 additional information the enzyme is involved in fructan degradation Cichorium intybus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.80 1-FEH
-
 Cichorium intybus
3.2.1.153 1-FEH IIa
-
 Cichorium intybus
3.2.1.153 fructan 1-exohydrolase
-
 Cichorium intybus