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Literature summary extracted from

  • Milkowski, C.; Baumert, A.; Schmidt, D.; Nehlin, L.; Strack, D.
    Molecular regulation of sinapate ester metabolism in Brassica napus: expression of genes, properties of the encoded proteins and correlation of enzyme activities with metabolite accumulation (2004), Plant J., 38, 80-92.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.91 nutrition in crop plants, sinapate esters are antinutritive compounds. They contribute to the bitter taste and astringency of seed products. Sinapate esters form complexes with proteins during seed oil processing, thus compromising the use of the valuable seed meal for animal feed and preventing it from being used as human food supplement. Thus, there is a fundamental interest in reducing the amount of sinapate esters in the seed. Suppressing the expression of the key enzymes in sinapine synthesis, sinapoylglucose:choline O-napoyltransferase (BnSCT) and UDP-glucose:sinapate glucosyltransferase (BnSGT1), by techniques such as dsRNAi should by a valuable step in establishing Brassica napus, an important oil crop, as a protein crop as well Brassica napus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.91 expression in Escherichia coli, the protein is exclusively present in inclusion bodies Brassica napus
2.3.1.92 expression in Escherichia coli, the protein is exclusively present in inclusion bodies Brassica napus
2.4.1.120 genomic organization analysis, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Brassica napus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.120 additional information
-
 additional information kinetic data for unpurified enzyme in crude protein extract of Escherichia coli expressing the recombinant enzyme Brassica napus
2.4.1.120 0.72
-
 sinapate
-
 Brassica napus
2.4.1.120 1
-
 UDP-glucose
-
 Brassica napus
2.4.1.120 1.7
-
 ferulate
-
 Brassica napus
2.4.1.120 2.3
-
 caffeate
-
 Brassica napus
2.4.1.120 3.6
-
 4-coumarate
-
 Brassica napus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.91 inclusion body the recombinant enzyme, expressed in Escherichia coli is exclusively present in inclusion bodies Brassica napus 16234
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.120 50270
-
 x * 55970, unmodified enzyme, amino acid sequence calculation, x * 50270, mature enzyme, amino acid sequence calculation Brassica napus
2.4.1.120 55970
-
 x * 55970, unmodified enzyme, amino acid sequence calculation, x * 50270, mature enzyme, amino acid sequence calculation Brassica napus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.120 UDP-alpha-D-glucose + sinapate Brassica napus regulation of the sinapate ester metabolism, enzyme expression is restricted to developing seeds, where it is involved in accumulation kinetics of corresponding metabolites UDP + 1-O-sinapoyl-beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.91 Brassica napus
-
 L. cv. Express
-
2.3.1.92 Brassica napus
-
 L. cv. Express
-
2.4.1.120 Brassica napus Q9FYU7
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.120 glycoprotein enzyme is putatively N-glycosylated in the Golgi apparatus Brassica napus
2.4.1.120 proteolytic modification enzyme contains a signal peptide, cleavage probably after residue D19 Brassica napus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.120 UDP-alpha-D-glucose + sinapate = UDP + 1-O-sinapoyl-beta-D-glucose random bi bi mechanism Brassica napus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.120 additional information expression analysis during seed development, accumulation of BnSGT1 transcripts and sinapate esters at early cotyledonary stages, overview Brassica napus
-
2.4.1.120 seed transcription levels increase throughout early stages of seed development until the early- and mid-cotyledonary stage, and stays constant in later stages Brassica napus
-
2.4.1.120 seedling highest expression level at age of 2 days, followed by a dramatic decrease Brassica napus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.120 UDP-alpha-D-glucose + 4-coumarate recombinant enzyme, 2% efficiency compared to sinapate Brassica napus UDP + 1-O-(4-coumaroyl)-beta-D-glucose
-
 ?
2.4.1.120 UDP-alpha-D-glucose + ferulate recombinant enzyme, 4% efficiency compared to sinapate Brassica napus UDP + 1-O-feruloyl-beta-D-glucose
-
 ?
2.4.1.120 UDP-alpha-D-glucose + sinapate regulation of the sinapate ester metabolism, enzyme expression is restricted to developing seeds, where it is involved in accumulation kinetics of corresponding metabolites Brassica napus UDP + 1-O-sinapoyl-beta-D-glucose
-
 ?
2.4.1.120 UDP-alpha-D-glucose + sinapate recombinant enzyme, best substrate Brassica napus UDP + 1-O-sinapoyl-beta-D-glucose
-
 ?
2.4.1.120 UDP-glucose + caffeate recombinant enzyme, 1% efficiency compared to sinapate Brassica napus UDP + 1-caffeoyl-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.120 ? x * 55970, unmodified enzyme, amino acid sequence calculation, x * 50270, mature enzyme, amino acid sequence calculation Brassica napus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.91 BnSCT
-
 Brassica napus
2.3.1.91 sinapoylglucose:B. napus choline sinapoyltransferase
-
 Brassica napus
2.3.1.92 sinapoylglucose:malate sinapoyltransferase
-
 Brassica napus
2.3.1.92 SMT
-
 Brassica napus
2.4.1.120 BnSGT1
-
 Brassica napus
2.4.1.120 More enzyme belongs to the serine carboxypeptidase-like family of acyltransferases Brassica napus
2.4.1.120 UDP-Glc:B. napus sinapate glucosyltransferase
-
 Brassica napus
2.4.1.120 UDP-glucose:B. napus sinapate glucosyltransferase
-
 Brassica napus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.120 30
-
 assay at Brassica napus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.120 6
-
 assay at Brassica napus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.120 Brassica napus sequence calculation
-
 6.1
2.4.1.120 Brassica napus sequence calculation, mature protein
-
 6.6