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Literature summary extracted from

  • Tsakraklides, G.; Martin, M.; Chalam, R.; Tarczynski, M.C.; Schmidt, A.; Leustek, T.
    Sulfate reduction is increased in transgenic Arabidopsis thaliana expressing 5'-adenylylsulfate reductase from Pseudomonas aeruginosa (2002), Plant J., 32, 879-889.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.4.9 additional information feeding of O-acetylserine leads to increased accumulation of sulfite and thiosulfate in wild-type plants Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.9 recombinant expression of the enzyme, fused to the transit peptide rbcS, in Arabidopsis thaliana under transcriptional control of the CaMV 35S promotor Pseudomonas aeruginosa
1.8.99.2 localized expression in plastids of Arabidopsis thaliana, transgenic plants accumulate sulfite, thiosulfate, cysteine and gluthatione Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.9 additional information construction of transgenic Arabidopsis thaliana plants expressing the active enzyme from Pseudomonas aeruginosa in the chloroplasts, the plants show reduced fresh and dry weight, delayed germination, smaller leaves, slightly reduced fertility, and increased enzyme activity and sulfate reduction accumulating of sulfite, thiosulfate, cysteine, gamma-glutamylcysteine, and glutathione, feeding of O-acetylserine leads to increased accumulation of sulfite and thiosulfate in the transgenic plants, phenotype alterations, overview Arabidopsis thaliana
1.8.4.9 additional information construction of transgenic Arabidopsis thaliana plants expressing the active enzyme from Pseudomonas aeruginosa in the chloroplasts, the plants show reduced fresh and dry weight, delayed germination, smaller leaves, slightly reduced fertility, and increased enzyme activity and sulfate reduction accumulating of sulfite, thiosulfate, cysteine, gamma-glutamylcysteine, and glutathione, feeding of O-acetylserine leads to increased accumulation of sulfite and thiosulfate in the transgenic plants, phenotype alterations, overview Pseudomonas aeruginosa

General Stability

EC Number General Stability Organism
1.8.4.9 enzyme is highly stable in vitro Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.4.9 29000
-
 x * 29000, recombinant enzyme, SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.9 5'-adenylyl sulfate + glutathione Arabidopsis thaliana enzyme is involved in reductive sulfate assimilation, pathway overview AMP + sulfite + glutathione disulfide
-
 ?
1.8.4.9 5'-adenylyl sulfate + glutathione Pseudomonas aeruginosa enzyme is involved in reductive sulfate assimilation, pathway overview AMP + sulfite + glutathione disulfide
-
 ?
1.8.4.9 5'-adenylyl sulfate + thioredoxin Pseudomonas aeruginosa recombinant enzyme in transgenic Arabidopsis thaliana plants AMP + sulfite + thioredoxin disulfide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.9 Arabidopsis thaliana
-
-
-
1.8.4.9 Pseudomonas aeruginosa
-
-
-
1.8.99.2 Pseudomonas aeruginosa
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.4.9 0.00077
-
 wild-type plants Arabidopsis thaliana
1.8.4.9 0.0023 0.006 recombinant enzyme in transgenic Arabidopsis thaliana plants, cofactor thioredoxin Pseudomonas aeruginosa
1.8.4.9 0.0023 0.006 transgenic plants with the recombinant Pseudomonas aeruginosa enzyme utilizing thioredoxin Arabidopsis thaliana
1.8.99.2 additional information
-
 specific activities in transgenic Arabidopsis thaliana shoots transfected with Pseudomonas aeruginosa adenylyl-sulfate reductase Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.9 5'-adenylyl sulfate + glutathione
-
 Arabidopsis thaliana AMP + sulfite + glutathione disulfide
-
 ?
1.8.4.9 5'-adenylyl sulfate + glutathione
-
 Pseudomonas aeruginosa AMP + sulfite + glutathione disulfide
-
 ?
1.8.4.9 5'-adenylyl sulfate + glutathione enzyme is involved in reductive sulfate assimilation, pathway overview Arabidopsis thaliana AMP + sulfite + glutathione disulfide
-
 ?
1.8.4.9 5'-adenylyl sulfate + glutathione enzyme is involved in reductive sulfate assimilation, pathway overview Pseudomonas aeruginosa AMP + sulfite + glutathione disulfide
-
 ?
1.8.4.9 5'-adenylyl sulfate + thioredoxin recombinant enzyme in transgenic Arabidopsis thaliana plants Pseudomonas aeruginosa AMP + sulfite + thioredoxin disulfide
-
 ?
1.8.99.2 adenylyl sulfate + reduced thioredoxin 1
-
 Pseudomonas aeruginosa AMP + sulfite + oxidized thioredoxin 1
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.4.9 ? x * 29000, recombinant enzyme, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
1.8.4.9 5'-adenylylsulfate reductase
-
 Arabidopsis thaliana
1.8.4.9 5'-adenylylsulfate reductase
-
 Pseudomonas aeruginosa
1.8.4.9 APR
-
 Arabidopsis thaliana
1.8.4.9 APR
-
 Pseudomonas aeruginosa
1.8.4.9 APS reductase
-
 Arabidopsis thaliana
1.8.4.9 APS reductase
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.9 glutathione
-
 Arabidopsis thaliana
1.8.4.9 glutathione
-
 Pseudomonas aeruginosa
1.8.4.9 additional information no activity with thioredoxin Arabidopsis thaliana
1.8.4.9 thioredoxin recombinant enzyme utilizes the plant thioredoxins m and f in transgenic plants Pseudomonas aeruginosa