Any feedback?
Literature summary extracted from
- A peptide methionine sulfoxide reductase highly expressed in photosynthetic tissue in Arabidopsis thaliana can protect the chaperone-like activity of a chloroplast-localized small heat shock protein (2002), Plant J., 29, 545-553.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.8.4.11 | biotechnology | enzyme is a target for modification of redox-dependent regulation | Arabidopsis thaliana |
| 1.8.4.12 | biotechnology | enzyme is a target for modification of redox-dependent regulation | Arabidopsis thaliana |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.4.11 | expression of the plastidic isozyme pPMSR in Escherichia coli strain BL21(DE3) without the chloroplast signal sequence | Arabidopsis thaliana |
| 1.8.4.12 | expression of the plastidic isozyme pPMSR in Escherichia coli strain BL21(DE3) without the chloroplast signal sequence | Arabidopsis thaliana |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.8.4.11 | C151A | site-directed mutagenesis, activity with Hsp21 is similar to the wild-type enzyme | Arabidopsis thaliana |
| 1.8.4.12 | C151A | site-directed mutagenesis, activity with Hsp21 is similar to the wild-type enzyme | Arabidopsis thaliana |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.8.4.11 | chloroplast | plastidic isozyme pPMSR | Arabidopsis thaliana | 9507 | - |
| 1.8.4.12 | chloroplast | plastidic isozyme pPMSR | Arabidopsis thaliana | 9507 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.11 | Hsp21 L-methionine S-oxide + dithiothreitol | Arabidopsis thaliana | chloroplast-localized small heat shock protein, repair function for heat shock protein Hsp21 by restoring the structure, which is crucial for cellular resistance to oxidative stress, the enzyme can protect the chaperone-like activity of Hsp21 | Hsp21 L-methionine + dithiothreitol S-oxide | - |
? |  |
| 1.8.4.11 | additional information | Arabidopsis thaliana | enzyme has regulatory function in the plant cell | ? | - |
? | |
| 1.8.4.12 | Hsp21 L-methionine S-oxide + dithiothreitol | Arabidopsis thaliana | chloroplast-localized small heat shock protein, repair function for heat shock protein Hsp21 by restoring the structure, which is crucial for cellular resistance to oxidative stress, the enzyme can protect the chaperone-like activity of Hsp21 | Hsp21 L-methionine + dithiothreitol S-oxide | - |
? | |
| 1.8.4.12 | additional information | Arabidopsis thaliana | enzyme has regulatory function in the plant cell | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.4.11 | Arabidopsis thaliana | - |
5 genes, including 1 plastidic isozyme | - |
| 1.8.4.12 | Arabidopsis thaliana | - |
5 genes, including 1 plastidic isozyme | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.4.11 | recombinant plastidic isozyme pPMSR from Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
| 1.8.4.12 | recombinant plastidic isozyme pPMSR from Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.8.4.11 | additional information | high expression level of the plastidic isozyme pPMSR in photosynthetic active tissue | Arabidopsis thaliana | - |
| 1.8.4.12 | additional information | high expression level of the plastidic isozyme pPMSR in photosynthetic active tissue | Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.11 | Hsp21 L-methionine S-oxide + dithiothreitol | chloroplast-localized small heat shock protein, repair function for heat shock protein Hsp21 by restoring the structure, which is crucial for cellular resistance to oxidative stress, the enzyme can protect the chaperone-like activity of Hsp21 | Arabidopsis thaliana | Hsp21 L-methionine + dithiothreitol S-oxide | - |
? | |
| 1.8.4.11 | Hsp21 L-methionine S-oxide + dithiothreitol | Hsp21 contains 6 methionine residues at positions 49, 52, 55, 59, 62, and 67, about half of the residues are reduced by the enzyme probably due to its stereospecificity | Arabidopsis thaliana | Hsp21 L-methionine + dithiothreitol S-oxide | - |
? | |
| 1.8.4.11 | additional information | enzyme has regulatory function in the plant cell | Arabidopsis thaliana | ? | - |
? | |
| 1.8.4.12 | Hsp21 L-methionine S-oxide + dithiothreitol | chloroplast-localized small heat shock protein, repair function for heat shock protein Hsp21 by restoring the structure, which is crucial for cellular resistance to oxidative stress, the enzyme can protect the chaperone-like activity of Hsp21 | Arabidopsis thaliana | Hsp21 L-methionine + dithiothreitol S-oxide | - |
? | |
| 1.8.4.12 | Hsp21 L-methionine S-oxide + dithiothreitol | Hsp21 contains 6 methionine residues at positions 49, 52, 55, 59, 62, and 67, about half of the residues are reduced by the enzyme probably due to its stereospecificity | Arabidopsis thaliana | Hsp21 L-methionine + dithiothreitol S-oxide | - |
? | |
| 1.8.4.12 | additional information | enzyme has regulatory function in the plant cell | Arabidopsis thaliana | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.4.11 | peptide methionine sulfoxide reductase | - |
Arabidopsis thaliana |
| 1.8.4.11 | PMSR | - |
Arabidopsis thaliana |
| 1.8.4.12 | peptide methionine sulfoxide reductase | - |
Arabidopsis thaliana |
| 1.8.4.12 | PMSR | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 25 | - |
assay at | Arabidopsis thaliana |
| 1.8.4.12 | 25 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 7.8 | - |
assay at | Arabidopsis thaliana |
| 1.8.4.12 | 7.8 | - |
assay at | Arabidopsis thaliana |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.11 | dithiothreitol | absolutely dependent on in vitro and in vivo with substrate Hsp21 | Arabidopsis thaliana | |
| 1.8.4.12 | dithiothreitol | absolutely dependent on in vitro and in vivo with substrate Hsp21 | Arabidopsis thaliana | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
