The in vitro substrate regiospecificity of recombinant UGT85B1, the cyanohydrin glucosyltransferase from Sorghum bicolor
Hansen, K.S.; Kristensen, C.; Tattersall, D.B.; Jones, P.R.; Olsen, C.E.; Bak, S.; Moller, B.L.; Phytochemistry 64, 143-151 (2003) 
Data extracted from this reference:
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.85
0.13
-
beta-citronellol
-
Sorghum bicolor
2.4.1.85
0.14
-
geraniol
-
Sorghum bicolor
2.4.1.85
0.66
-
1-Hexanol
-
Sorghum bicolor
2.4.1.85
0.69
-
3-methyl-3-buten-1-ol
-
Sorghum bicolor
2.4.1.85
0.73
-
cis-3-hexen-1-ol
-
Sorghum bicolor
2.4.1.85
0.84
-
mandelonitrile
-
Sorghum bicolor
2.4.1.85
1.13
-
nerol
-
Sorghum bicolor
2.4.1.85
6.33
-
2-hydroxy-3-methoxybenzyl alcohol
-
Sorghum bicolor
2.4.1.85
10.3
-
3-methyl-2-buten-1-ol
-
Sorghum bicolor
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.4.1.85
Sorghum bicolor
-
recombinant enzyme from Escherichia coli
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.4.1.85
UDP-glucose + 1-hexanol
-
660095
Sorghum bicolor
UDP + hexyl beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + 2-hydroxy-2-methylbutyronitrile
-
660095
Sorghum bicolor
UDP + ?
-
-
-
?
2.4.1.85
UDP-glucose + 2-hydroxy-3-methoxybenzyl alcohol
-
660095
Sorghum bicolor
UDP + ?
-
-
-
?
2.4.1.85
UDP-glucose + 3-methyl-2-buten-1-ol
-
660095
Sorghum bicolor
UDP + 3-methyl-2-butenyl-beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + 3-methyl-3-buten-1-ol
-
660095
Sorghum bicolor
UDP + 3-methyl-3-butenyl-beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + 4-hydroxymandelonitrile
-
660095
Sorghum bicolor
UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + acetone cyanohydrin
-
660095
Sorghum bicolor
UDP + ?
-
-
-
?
2.4.1.85
UDP-glucose + alpha-terpinol
-
660095
Sorghum bicolor
UDP + alpha-terpinyl-beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + beta-citronellol
-
660095
Sorghum bicolor
UDP + beta-citronellol beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + cis-3-hexen-1-ol
-
660095
Sorghum bicolor
UDP + cis-3-hexenyl-beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + farnesol
-
660095
Sorghum bicolor
UDP + farnesyl beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + geraniol
-
660095
Sorghum bicolor
UDP + geranyl-beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + linalool
-
660095
Sorghum bicolor
UDP + linalool beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + mandelonitrile
-
660095
Sorghum bicolor
UDP + 2-(beta-D-glucopyranosyl)-2-phenylacetonitrile
-
-
-
?
2.4.1.85
UDP-glucose + nerol
-
660095
Sorghum bicolor
UDP + nerol beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + trans-2-hexen-1-ol
-
660095
Sorghum bicolor
UDP + trans-2-hexenyl-beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + vanillic acid
-
660095
Sorghum bicolor
UDP + vanillic acid beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + vanillin
-
660095
Sorghum bicolor
UDP + vanillin beta-D-glucoside
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
2.4.1.85
cyanohydrin glucosyltransferase
-
Sorghum bicolor
2.4.1.85
UDP-glucose:p-hydroxymandelonitrile-O-glucosyltransferase
-
Sorghum bicolor
2.4.1.85
UGT85B1
-
Sorghum bicolor
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.4.1.85
0.03
-
cis-3-hexen-1-ol
-
Sorghum bicolor
2.4.1.85
0.06
-
1-Hexanol
-
Sorghum bicolor
2.4.1.85
0.06
-
nerol
-
Sorghum bicolor
2.4.1.85
0.07
-
3-methyl-3-buten-1-ol
-
Sorghum bicolor
2.4.1.85
0.09
-
beta-citronellol
-
Sorghum bicolor
2.4.1.85
0.1
-
geraniol
-
Sorghum bicolor
2.4.1.85
0.39
-
2-hydroxy-3-methoxybenzyl alcohol
-
Sorghum bicolor
2.4.1.85
0.91
-
3-methyl-2-buten-1-ol
-
Sorghum bicolor
2.4.1.85
10.6
-
mandelonitrile
-
Sorghum bicolor
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.85
0.13
-
beta-citronellol
-
Sorghum bicolor
2.4.1.85
0.14
-
geraniol
-
Sorghum bicolor
2.4.1.85
0.66
-
1-Hexanol
-
Sorghum bicolor
2.4.1.85
0.69
-
3-methyl-3-buten-1-ol
-
Sorghum bicolor
2.4.1.85
0.73
-
cis-3-hexen-1-ol
-
Sorghum bicolor
2.4.1.85
0.84
-
mandelonitrile
-
Sorghum bicolor
2.4.1.85
1.13
-
nerol
-
Sorghum bicolor
2.4.1.85
6.33
-
2-hydroxy-3-methoxybenzyl alcohol
-
Sorghum bicolor
2.4.1.85
10.3
-
3-methyl-2-buten-1-ol
-
Sorghum bicolor
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.4.1.85
UDP-glucose + 1-hexanol
-
660095
Sorghum bicolor
UDP + hexyl beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + 2-hydroxy-2-methylbutyronitrile
-
660095
Sorghum bicolor
UDP + ?
-
-
-
?
2.4.1.85
UDP-glucose + 2-hydroxy-3-methoxybenzyl alcohol
-
660095
Sorghum bicolor
UDP + ?
-
-
-
?
2.4.1.85
UDP-glucose + 3-methyl-2-buten-1-ol
-
660095
Sorghum bicolor
UDP + 3-methyl-2-butenyl-beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + 3-methyl-3-buten-1-ol
-
660095
Sorghum bicolor
UDP + 3-methyl-3-butenyl-beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + 4-hydroxymandelonitrile
-
660095
Sorghum bicolor
UDP + (S)-4-hydroxymandelonitrile beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + acetone cyanohydrin
-
660095
Sorghum bicolor
UDP + ?
-
-
-
?
2.4.1.85
UDP-glucose + alpha-terpinol
-
660095
Sorghum bicolor
UDP + alpha-terpinyl-beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + beta-citronellol
-
660095
Sorghum bicolor
UDP + beta-citronellol beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + cis-3-hexen-1-ol
-
660095
Sorghum bicolor
UDP + cis-3-hexenyl-beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + farnesol
-
660095
Sorghum bicolor
UDP + farnesyl beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + geraniol
-
660095
Sorghum bicolor
UDP + geranyl-beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + linalool
-
660095
Sorghum bicolor
UDP + linalool beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + mandelonitrile
-
660095
Sorghum bicolor
UDP + 2-(beta-D-glucopyranosyl)-2-phenylacetonitrile
-
-
-
?
2.4.1.85
UDP-glucose + nerol
-
660095
Sorghum bicolor
UDP + nerol beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + trans-2-hexen-1-ol
-
660095
Sorghum bicolor
UDP + trans-2-hexenyl-beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + vanillic acid
-
660095
Sorghum bicolor
UDP + vanillic acid beta-D-glucoside
-
-
-
?
2.4.1.85
UDP-glucose + vanillin
-
660095
Sorghum bicolor
UDP + vanillin beta-D-glucoside
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.4.1.85
0.03
-
cis-3-hexen-1-ol
-
Sorghum bicolor
2.4.1.85
0.06
-
1-Hexanol
-
Sorghum bicolor
2.4.1.85
0.06
-
nerol
-
Sorghum bicolor
2.4.1.85
0.07
-
3-methyl-3-buten-1-ol
-
Sorghum bicolor
2.4.1.85
0.09
-
beta-citronellol
-
Sorghum bicolor
2.4.1.85
0.1
-
geraniol
-
Sorghum bicolor
2.4.1.85
0.39
-
2-hydroxy-3-methoxybenzyl alcohol
-
Sorghum bicolor
2.4.1.85
0.91
-
3-methyl-2-buten-1-ol
-
Sorghum bicolor
2.4.1.85
10.6
-
mandelonitrile
-
Sorghum bicolor