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Literature summary extracted from

  • Duarte-Vazquez, M.A.; Garcia-Almendarez, B.E.; Rojo-Dominguez, A.; Whitaker, J.R.; Arroyave-Hernandez, C.; Regalado, C.
    Monosaccharide composition and properties of a deglycosylated turnip peroxidase isozyme (2003), Phytochemistry, 62, 5-11.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.11.1.7 a 10% and 74% decrease in activity of native and deglycosylated peroxidase, respectively, is observed after 3 h incubation with trypsin Brassica napus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.7 0.025
-
 H2O2 25°C, pH 6.0, partially deglycosylated enzyme Brassica napus
1.11.1.7 0.055
-
 H2O2 25°C, pH 6.0, native enzyme Brassica napus
1.11.1.7 0.9
-
 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) 25°C, pH 6.0, partially deglycosylated enzyme Brassica napus
1.11.1.7 1.1
-
 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) 25°C, pH 6.0, native enzyme Brassica napus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Brassica napus
-
 turnip
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.11.1.7 glycoprotein 9.1% carbohydrate content, intact oligosaccacharide chains are required for full peroxidase activity Brassica napus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.7 cation exchange, hydrophobic interaction chromatography Brassica napus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.7 root
-
 Brassica napus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O2
-
 Brassica napus oxidized 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.7 65
-
 approx. 50% loss of native and deglycosylated peroxidase activity after 3 min and 1 min, respectively Brassica napus