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Literature summary extracted from

  • Chareonthiphakorn, N.; Wititsuwannakul, D.; Golan-Goldhirsh, A.; Wititsuwannakul, R.
    Purification and characterization of NAD(P)H quinone reductase from the latex of Hevea brasiliensis Müll.-Arg. (Euphorbiaceae) (2002), Phytochemistry, 61, 123-128.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.2 0.2
-
 NADH pH 8.0, reaction with plumbagin Hevea brasiliensis
1.6.5.2 0.204
-
 NADH pH 8.0, reaction with menadione Hevea brasiliensis
1.6.5.2 0.25
-
 NADH pH 8.0, reaction with juglone Hevea brasiliensis
1.6.5.2 0.27
-
 NADPH pH 8.0, reaction with plumbagin Hevea brasiliensis
1.6.5.2 0.286
-
 NADPH pH 8.0, reaction with juglone Hevea brasiliensis
1.6.5.2 0.322
-
 NADPH pH 8.0, reaction with menadione Hevea brasiliensis
1.6.5.2 0.333
-
 NADH pH 8.0, reaction with p-benzoquinone Hevea brasiliensis
1.6.5.2 0.375
-
 NADH pH 8.0, reaction with duroquinone Hevea brasiliensis
1.6.5.2 0.384
-
 NADPH pH 8.0, reaction with p-benzoquinone Hevea brasiliensis
1.6.5.2 0.435
-
 duroquinone pH 8.0, reaction with duroquinone Hevea brasiliensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.2 21000
-
 4 * 21000, SDS-PAGE Hevea brasiliensis
1.6.5.2 83000
-
 gel filtration Hevea brasiliensis

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.2 Hevea brasiliensis
-
 Müll.-Arg.
-
1.6.5.2 Hevea brasiliensis Mull.-Arg.
-
 Müll.-Arg.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.2
-
 Hevea brasiliensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.5.2 latex
-
 Hevea brasiliensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.5.2 1.23
-
-
 Hevea brasiliensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.2 2-methyl-1,4-naphthoquinone + NADH
-
 Hevea brasiliensis 2-methyl-1,4-naphthoquinol + NAD+
-
 ?
1.6.5.2 2-methyl-1,4-naphthoquinone + NADH
-
 Hevea brasiliensis Mull.-Arg. 2-methyl-1,4-naphthoquinol + NAD+
-
 ?
1.6.5.2 2-methyl-1,4-naphthoquinone + NADPH
-
 Hevea brasiliensis 2-methyl-1,4-naphthoquinol + NADp+
-
 ?
1.6.5.2 2-methyl-1,4-naphthoquinone + NADPH
-
 Hevea brasiliensis Mull.-Arg. 2-methyl-1,4-naphthoquinol + NADp+
-
 ?
1.6.5.2 duroquinone + NADH
-
 Hevea brasiliensis ? + NAD+
-
 ?
1.6.5.2 duroquinone + NADH
-
 Hevea brasiliensis Mull.-Arg. ? + NAD+
-
 ?
1.6.5.2 duroquinone + NADPH
-
 Hevea brasiliensis ? + NADP+
-
 ?
1.6.5.2 duroquinone + NADPH
-
 Hevea brasiliensis Mull.-Arg. ? + NADP+
-
 ?
1.6.5.2 juglone + NADH
-
 Hevea brasiliensis dihydrojuglone + NAD+
-
 ?
1.6.5.2 juglone + NADH
-
 Hevea brasiliensis Mull.-Arg. dihydrojuglone + NAD+
-
 ?
1.6.5.2 juglone + NADPH
-
 Hevea brasiliensis dihydrojuglone + NADP+
-
 ?
1.6.5.2 p-benzoquinone + NADH
-
 Hevea brasiliensis benzohydroquinone + NAD+
-
 ?
1.6.5.2 p-benzoquinone + NADPH
-
 Hevea brasiliensis p-benzohydroquinone + NADP+
-
 ?
1.6.5.2 plumbagin + NADH
-
 Hevea brasiliensis ? + NAD+
-
 ?
1.6.5.2 plumbagin + NADPH
-
 Hevea brasiliensis ? + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.5.2 tetramer 4 * 21000, SDS-PAGE Hevea brasiliensis

Synonyms

EC Number Synonyms Comment Organism
1.6.5.2 NAD(P)H quinone reductase
-
 Hevea brasiliensis
1.6.5.2 NAD(P)H-QR
-
 Hevea brasiliensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.5.2 80
-
 10 h, more than 70% of the latex enzyme activity is retained Hevea brasiliensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.5.2 8
-
-
 Hevea brasiliensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.5.2 5 9 pH 5.0: about 45% of maximal activity, pH 9.0: about 55% of maximal activity Hevea brasiliensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.2 NADH
-
 Hevea brasiliensis
1.6.5.2 NADPH
-
 Hevea brasiliensis