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Literature summary extracted from

  • Gisselsson, A.; Szilagyi, A.; Akerlund, H.E.
    Role of histidines in the binding of violaxanthin de-epoxidase to the thylakoid membrane as studied by site-directed mutagenesis (2004), Physiol. Plant., 122, 337-343.
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
1.23.5.1 H121A/H124A considerably lower pH dependence for binding than wild-type, cooperativity value around 2 compared to wild-type value of 3.7. Km-value for ascorbate is 3.2 mM compared to 1.9 mM for wild-type enzyme Spinacia oleracea
1.23.5.1 H124R considerably lower pH dependence for binding than wild-type, cooperativity value around 2 compared to wild-type value of 3.7. Km-value for ascorbate is 2.1 mM compared to 1.9 mM for wild-type enzyme Spinacia oleracea
1.23.5.1 H124R considerably lower pH dependence for binding than wild-type, cooperativity value around 2 compared to wild-type value of 3.7.Km-value for ascorbate is 1.5 mM compared to 1.9 mM for wild-type enzyme Spinacia oleracea
1.23.5.1 H134A considerably lower pH dependence for binding than wild-type, cooperativity value around 2 compared to wild-type value of 3.7 Spinacia oleracea
1.23.5.1 H167A/H173A considerably lower pH dependence for binding than wild-type, cooperativity value of 1.6 compared to wild-type value of 3.7. Km-value for ascorbate is 8.3 mM compared to 1.9 mM for wild-type enzyme Spinacia oleracea
1.23.5.1 H167R/H173R considerably lower pH dependence for binding than wild-type, cooperativity value around 2 compared to wild-type value of 3.7. Km-value for ascorbate is 6.3 mM compared to 1.9 mM for wild-type enzyme Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.23.5.1 thylakoid regulation of the violaxanthin de-epoxidase involves a conformational change at low lumenal pH, followed by binding of the enzyme to the thylakoid membrane. Protonation of His at low pH induces the conformational change of the enzyme, and hence indirectly regulates binding of the enzyme to the thylakoid membrane Triticum aestivum 9579
-
1.23.5.1 thylakoid regulation of the violaxanthin de-epoxidase involves a conformational change at low lumenal pH, followed by binding of the enzyme to the thylakoid membrane. Protonation of His at low pH induces the conformational change of the enzyme, and hence indirectly regulates binding of the enzyme to the thylakoid membrane Spinacia oleracea 9579
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.23.5.1 antheraxanthin + ascorbate Triticum aestivum
-
 zeaxanthin + dehydroascorbate + H2O
-
 ?
1.23.5.1 antheraxanthin + ascorbate Spinacia oleracea
-
 zeaxanthin + dehydroascorbate + H2O
-
 ?
1.23.5.1 violaxanthin + ascorbate Triticum aestivum
-
 antheraxanthin + dehydroascorbate + H2O
-
 ?
1.23.5.1 violaxanthin + ascorbate Spinacia oleracea
-
 antheraxanthin + dehydroascorbate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.23.5.1 Spinacia oleracea
-
-
-
1.23.5.1 Triticum aestivum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.23.5.1 antheraxanthin + ascorbate
-
 Triticum aestivum zeaxanthin + dehydroascorbate + H2O
-
 ?
1.23.5.1 antheraxanthin + ascorbate
-
 Spinacia oleracea zeaxanthin + dehydroascorbate + H2O
-
 ?
1.23.5.1 violaxanthin + ascorbate
-
 Triticum aestivum antheraxanthin + dehydroascorbate + H2O
-
 ?
1.23.5.1 violaxanthin + ascorbate
-
 Spinacia oleracea antheraxanthin + dehydroascorbate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.23.5.1 VDE
-
 Triticum aestivum
1.23.5.1 VDE
-
 Spinacia oleracea