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Literature summary extracted from

  • Low, J.C.; Tu, S.C.
    Energy transfer evidence for in vitro and in vivo complexes of Vibrio harveyi flavin reductase P and luciferase (2003), Photochem. Photobiol., 77, 446-452.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.30 gene frp, functional expression of the enzyme fused to yellow fluorescence protein YFP in a Vibrio harveyi strain lacking the frp gene, subcloning and expression in Escherichia coli strains JM109 and BL21(DE3), the yellow fluorescence protein YFP is a mutant of the green fluorescence protein GFP, with mutations S65G, V68L, S72A, and T203Y Vibrio harveyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.30 0.021
-
 FMN recombinant fusion protein, pH 7.0, 23°C Vibrio harveyi
1.5.1.30 0.048
-
 NADPH recombinant fusion protein, pH 7.0, 23°C Vibrio harveyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.30 53783
-
 x * 53783, recombinant fusion protein, sequence calculation Vibrio harveyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.30 additional information Vibrio harveyi enzyme transfers reduced riboflavin 5'-phosphate to luciferase by direct channeling in vitro and in vivo, formation of donor-acceptor enzyme complex ?
-
 ?
1.5.1.30 riboflavin + NADPH Vibrio harveyi
-
 reduced riboflavin + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.30 Vibrio harveyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.30 recombinant enzyme from Escherichia coli strain BL21(DE3) to over 90% purity, recombinant fusion protein from Escherichia coli strain JM109 to over 80% purity Vibrio harveyi

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.1.30 reduced riboflavin + NADP+ = riboflavin + NADPH + H+ catalytic mechanism Vibrio harveyi
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.30 FMN + NADPH
-
 Vibrio harveyi FMNH2 + NADP+
-
 ?
1.5.1.30 additional information enzyme transfers reduced riboflavin 5'-phosphate to luciferase by direct channeling in vitro and in vivo, formation of donor-acceptor enzyme complex Vibrio harveyi ?
-
 ?
1.5.1.30 riboflavin + NADPH
-
 Vibrio harveyi reduced riboflavin + NADP+
-
 ?
1.5.1.30 riboflavin + NADPH i.e. FMN Vibrio harveyi reduced riboflavin + NADP+ i.e. FMNH2 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.30 ? x * 53783, recombinant fusion protein, sequence calculation Vibrio harveyi

Synonyms

EC Number Synonyms Comment Organism
1.5.1.30 flavin reductase P
-
 Vibrio harveyi
1.5.1.30 FRP
-
 Vibrio harveyi
1.5.1.30 More see also EC 1.5.1.29 Vibrio harveyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.30 23
-
 assay at Vibrio harveyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.30 7
-
 assay at Vibrio harveyi

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.30 NADPH
-
 Vibrio harveyi