Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zinoviev, V.V.; Yakishchik, S.I.; Evdokimov, A.A.; Malygin, E.G.; Hattman, S.
    Symmetry elements in DNA structure important for recognition/methylation by DNA [amino]-methyltransferases (2004), Nucleic Acids Res., 32, 3930-3934.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.72 additional information
-
 additional information detailed kinetics for diverse substrate duplexes and methylation sites Escherichia coli
2.1.1.72 additional information
-
 additional information detailed kinetics for diverse substrate duplexes and methylation sites Tequatrovirus T4
2.1.1.113 additional information
-
 additional information Km-values for several oligonucleotides Bacillus amyloliquefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Escherichia coli
-
 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Tequatrovirus T4
-
 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Escherichia coli
-
-
-
2.1.1.72 Tequatrovirus T4
-
-
-
2.1.1.113 Bacillus amyloliquefaciens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine
-
 Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine
-
 Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine specific for methylation site sequence GATC and structure, an intact GA sequence is essential for activity, altered structural symmetry of the DNA substrate decreases kcat sharply, the best contact between enzyme and DNA is a palindromic interaction site covering the 5'-symmetric residues, which is located in the major groove, and another one in the 3'-half covering the 3'-symmetric residues is located in the minor groove, overview Escherichia coli S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine specific for methylation site sequence GATC and structure, an intact GAT sequence is essential for activity, altered structural symmetry of the DNA substrate decreases kcat sharply, the best contact between enzyme and DNA is a palindromic interaction site covering the 5'-symmetric residues, which is located in the major groove, and another one in the 3'-half covering the 3'-symmetric residues is located in the minor groove, overview Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?
2.1.1.113 additional information integrity of ATCC sequence is critical Bacillus amyloliquefaciens ?
-
 ?
2.1.1.113 S-adenosyl-L-methionine + [DNA]-cytosine
-
 Bacillus amyloliquefaciens S-adenosyl-L-homocysteine + [DNA]-N4-methylcytosine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 DNA [amino]-methyltransferase
-
 Escherichia coli
2.1.1.72 DNA [amino]-methyltransferase
-
 Tequatrovirus T4
2.1.1.72 DNA-[N6-adenine] MTase
-
 Escherichia coli
2.1.1.72 DNA-[N6-adenine] MTase
-
 Tequatrovirus T4
2.1.1.72 EcoDam
-
 Escherichia coli
2.1.1.72 EcoDam DNA-[N6-adenine] MTase
-
 Escherichia coli
2.1.1.72 T4Dam
-
 Tequatrovirus T4
2.1.1.72 T4Dam DNA-[N6-adenine] MTase
-
 Tequatrovirus T4
2.1.1.72 [N6-adenine] MTase
-
 Escherichia coli
2.1.1.72 [N6-adenine] MTase
-
 Tequatrovirus T4

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.72 25
-
 assay at Escherichia coli
2.1.1.72 25
-
 assay at Tequatrovirus T4

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.113 additional information
-
 additional information Kcat-values for several oligonucleotides Bacillus amyloliquefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.72 8
-
 assay at Escherichia coli
2.1.1.72 8
-
 assay at Tequatrovirus T4