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Literature summary extracted from

  • Droogmans, L.; Roovers, M.; Bujnicki, J.M.; Tricot, C.; Hartsch, T.; Stalon, V.; Grosjean, H.
    Cloning and characterization of tRNA (m1A58) methyltransferase (TrmI) from Thermus thermophilus HB27, a protein required for cell growth at extreme temperatures (2003), Nucleic Acids Res., 31, 2148-2156.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.220 expression in Escherichia coli Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.220 28582
-
 4 * 28582, calculation from sequence, composed of two types of subunits (Gcd14p and Gcd10p) Thermus thermophilus
2.1.1.220 150000
-
 gel filtration Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.220 Thermus thermophilus Q8GBB2
-
-
2.1.1.220 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 Q8GBB2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.220
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.220 S-adenosyl-L-methionine + adenine58 in tRNA catalyzes the sitespecific formation of m1A at position 58 of the T-loop of tRNA in the absence of any other complementary protein Thermus thermophilus S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
-
 ?
2.1.1.220 S-adenosyl-L-methionine + adenine58 in tRNA catalyzes the sitespecific formation of m1A at position 58 of the T-loop of tRNA in the absence of any other complementary protein Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.220 tetramer 4 * 28582, calculation from sequence, composed of two types of subunits (Gcd14p and Gcd10p) Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
2.1.1.220 TrmI
-
 Thermus thermophilus
2.1.1.220 tRNA (m1A58) methyltransferase
-
 Thermus thermophilus
2.1.1.220 tRNA (m1A58) MTase
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.220 60
-
 assay at Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.220 8
-
 assay at Thermus thermophilus

General Information

EC Number General Information Comment Organism
2.1.1.220 malfunction inactivation of the Thermus thermophilus trmI gene results in a thermosensitive phenotype (growth defect at 80°C) Thermus thermophilus
2.1.1.220 physiological function role of the N1-methylation of tRNA adenosine-58 in adaptation of life to extreme temperatures Thermus thermophilus