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Literature summary extracted from

  • Golser, R.; Gorren, A.C.; Mayer, B.; Schmidt, K.
    Functional characterization of Glu298Asp mutant human endothelial nitric oxide synthase purified from a yeast expression system (2003), Nitric Oxide, 8, 7-14.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.39 Calmodulin
-
 Homo sapiens
1.14.13.39 tetrahydrobiopterin
-
 Homo sapiens

Application

EC Number Application Comment Organism
1.14.13.39 medicine no evidence for altered enzyme function of mutant E298D that could explain endothelial dysfunction associated with the E298D polymorphism Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.39 E298D comparable to wild-type in heme and flavin content, in affinity to calmodulin and dimerization Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.39 0.0044
-
 L-arginine wild-type Homo sapiens
1.14.13.39 0.0052
-
 L-arginine mutant E298D Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Homo sapiens
-
 expression in Pichia pastoris
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 endothelium
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.39 0.142
-
 wild-type Homo sapiens
1.14.13.39 0.159
-
 mutant E298D Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Homo sapiens 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?