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Literature summary extracted from

  • Nagaraj, V.J.; Altenbach, D.; Galati, V.; Luescher, M.; Meyer, A.D.; Boller, T.; Wiemken, A.
    Distinct regulation of sucrose: sucrose-1-fructosyltransferase (1-SST) and sucrose: fructan-6-fructosyltransferase (6-SFT), the key enzymes of fructan synthesis in barley leaves: 1-SST as the pacemaker (2004), New Phytol., 161, 735-748.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.99
-
 Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.99 50000
-
 x * 61800, calculated, x * 50000 and x * 52000, SDS-PAGE Hordeum vulgare
2.4.1.99 52000
-
 x * 61800, calculated, x * 50000 and x * 52000, SDS-PAGE Hordeum vulgare
2.4.1.99 61800
-
 x * 61800, calculated, x * 50000 and x * 52000, SDS-PAGE Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.99 additional information Hordeum vulgare enzyme ins the pacemaker enzyme of fructan synthesis in barley leaves ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.99 Hordeum vulgare Q70LF5 expression in Pichia pastoris
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.99 glycoprotein contains six predicted glycosylation sites Hordeum vulgare
2.4.1.99 proteolytic modification
-
 Hordeum vulgare

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.99
-
 Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.99 1-kestose + 1-kestose
-
 Hordeum vulgare D-fructose + sucrose + ? and formation of some nystose ?
2.4.1.99 additional information enzyme ins the pacemaker enzyme of fructan synthesis in barley leaves Hordeum vulgare ?
-
 ?
2.4.1.99 sucrose + 1-kestose
-
 Hordeum vulgare D-glucose + ?
-
 ?
2.4.1.99 sucrose + sucrose
-
 Hordeum vulgare D-glucose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.99 ? x * 61800, calculated, x * 50000 and x * 52000, SDS-PAGE Hordeum vulgare
2.4.1.99 More enzyme is subject to rapid turnover Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
2.4.1.99 1-SST
-
 Hordeum vulgare

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.99 Hordeum vulgare calculated
-
 5.02