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Literature summary extracted from

  • Amiot, C.; Musard, J.F.; Hadjiyiassemis, M.; Jouvenot, M.; Fellmann, D.; Risold, P.Y.; Adami, P.
    Expression of the secreted FAD-dependent sulfhydryl oxidase (QSOX) in the guinea pig central nervous system (2004), Mol. Brain Res., 125, 13-21.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.3.2 gene cpQSOx1, primary gene sequence, DNA and amino acid sequence analysis, stable expression of the wild-type enzyme in MCF-7 cells with intra- and extracellular localization of the recombinant enzyme, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.3.2 extracellular enzyme is secreted Cavia porcellus
-
-
1.8.3.2 perikaryon
-
 Cavia porcellus 43204
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.3.2 68000
-
 x * 68000, recombinant His-tagged enzyme, SDS-PAGE Cavia porcellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.3.2 R-SH + O2 Cavia porcellus enzyme plays a role in synaptic strengthening and in redox activities in the brain R-S-S-R + H2O2
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.2 Cavia porcellus O08841
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.3.2 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel chelate chromatography Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.3.2 basal forebrain
-
 Cavia porcellus
-
1.8.3.2 brain detailed tissue distribution analysis in brain, overview Cavia porcellus
-
1.8.3.2 brain stem
-
 Cavia porcellus
-
1.8.3.2 central nervous system highest enzyme expression in reticular structures, such as the basal forebrain, reticular thalamic nucleus, and reticular nuclei of the brainstem Cavia porcellus
-
1.8.3.2 endometrium specific expression in glandular and luminal epithelial cells Cavia porcellus
-
1.8.3.2 epithelium secretory, endometrial Cavia porcellus
-
1.8.3.2 lung
-
 Cavia porcellus
-
1.8.3.2 neuron
-
 Cavia porcellus
-
1.8.3.2 ovary
-
 Cavia porcellus
-
1.8.3.2 secretory cell
-
 Cavia porcellus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.2 dithiothreitol + O2
-
 Cavia porcellus dithiothreitol disulfide + H2O2
-
 ir
1.8.3.2 R-SH + O2
-
 Cavia porcellus R-S-S-R + H2O2
-
 ir
1.8.3.2 R-SH + O2 enzyme plays a role in synaptic strengthening and in redox activities in the brain Cavia porcellus R-S-S-R + H2O2
-
 ir

Subunits

EC Number Subunits Comment Organism
1.8.3.2 ? x * 68000, recombinant His-tagged enzyme, SDS-PAGE Cavia porcellus

Synonyms

EC Number Synonyms Comment Organism
1.8.3.2 More enzyme belongs to the sulfhydryl oxidase/Quiescin Q6 family Cavia porcellus
1.8.3.2 QSOx1
-
 Cavia porcellus
1.8.3.2 sulfhydryl oxidase
-
 Cavia porcellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.3.2 37
-
 assay at Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.3.2 8
-
 assay at Cavia porcellus

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.3.2 FAD dependent on Cavia porcellus