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Literature summary extracted from

  • Panova, N.G.; Shcheveleva, E.V.; Alexeev, C.S.; Mukhortov, V.G.; Zuev, A.N.; Mikhailov, S.N.; Esipov, R.S.; Chuvikovsky, D.V.; Miroshnikov, A.I.
    Use of 4-thiouridine and 4-thiothymidine in studies on pyrimidine nucleoside phosphorylases (2004), Mol. Biol., 38, 770-776.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.2 expression of enzymes with uridine and thymidine phosphorylase activities in strain BL21(DE3) Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.2 additional information inhibitory effects between pyrimidines and analogues Escherichia coli
2.4.2.4 1-(2-hydroxyethoxymethyl)thymine
-
 Escherichia coli
2.4.2.4 2,2'-O-anhydrouridine
-
 Escherichia coli
2.4.2.4 5-fluoro-1-(tetrahydrofur-2-yl)uracil
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.2 additional information
-
 additional information kinetics Escherichia coli
2.4.2.2 0.13
-
 4-thiouridine pH 7.5, 25°C, uridine phosphorylase activity Escherichia coli
2.4.2.2 0.25
-
 4-thiothymidine pH 7.5, 25°C, thymidine phosphorylase activity Escherichia coli
2.4.2.4 0.25
-
 4-thiothymidine pH 7.5, 25°C Escherichia coli
2.4.2.4 0.3
-
 thymidine pH 7.5, 25°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.2 additional information Escherichia coli predictive profiling of 5-fluorouracil resistance in human cancer cells, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.2 Escherichia coli
-
-
-
2.4.2.4 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.2 recombinant enzymes Escherichia coli
2.4.2.4
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.2 45
-
 purified recombinant enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.2 4-thiothymidine + phosphate uridine phosphorylase activity, see also EC 2.4.2.3 Escherichia coli 4-thiothymine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 4-thiouridine + phosphate thymidine phosphorylase activity, see also EC 2.4.2.4 Escherichia coli 4-thiouracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.2 additional information predictive profiling of 5-fluorouracil resistance in human cancer cells, overview Escherichia coli ?
-
 ?
2.4.2.4 4-thiothymidine + phosphate
-
 Escherichia coli 4-thiothymine + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.4 thymidine + phosphate
-
 Escherichia coli thymine + 2-deoxy-D-ribose 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.2 pyrimidine nucleoside phosphorylase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.2 49
-
 4-thiouridine pH 7.5, 25°C, uridine phosphorylase activity Escherichia coli
2.4.2.2 63
-
 4-thiothymidine pH 7.5, 25°C, thymidine phosphorylase activity Escherichia coli
2.4.2.4 63
-
 4-thiothymidine pH 7.5, 25°C Escherichia coli
2.4.2.4 198
-
 thymidine pH 7.5, 25°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.2 7.5
-
 assay at Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.2 additional information
-
 additional information
-
 Escherichia coli
2.4.2.4 0.4
-
 2,2'-O-anhydrouridine pH 7.5, 25°C, activity with 4-thiothymidine Escherichia coli
2.4.2.4 0.4
-
 5-fluoro-1-(tetrahydrofur-2-yl)uracil pH 7.5, 25°C, activity with thymidine Escherichia coli
2.4.2.4 0.45
-
 5-fluoro-1-(tetrahydrofur-2-yl)uracil pH 7.5, 25°C, activity with 4-thiothymidine Escherichia coli
2.4.2.4 0.5
-
 2,2'-O-anhydrouridine pH 7.5, 25°C, activity with thymidine Escherichia coli
2.4.2.4 1.5
-
 1-(2-hydroxyethoxymethyl)thymine pH 7.5, 25°C, activity with 4-thiothymidine Escherichia coli