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Literature summary extracted from

  • Zinoviev, V.V.; Evdokimov, A.A.; Hattman, S.; Malygin, E.G.
    Molecular enzymology of phage T4 Dam DNA methyltransferase (2004), Mol. Biol., 38, 737-751.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.72 additional information
-
 additional information pre-steady-state and steady-state kinetics, reaction kinetics with diverse DNA duplex substrates, role of individual elements of the recognition site, kinetic reaction scheme assuming that the enzyme is isomerized into a catalytically active form, overview Tequatrovirus T4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.72 28000
-
 sucrose density gradient sedimentation Tequatrovirus T4
2.1.1.72 28400
-
 gel filtration Tequatrovirus T4
2.1.1.72 30690
-
 glycerol density gradient ultracentrifugation analysis Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine Tequatrovirus T4 hemimethylated DNA S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.72 Tequatrovirus T4
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.72 S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N6-methyladenine in DNA molecular mechanism, effector action of substrates, progressive methylation of extended DNA containing more than one methylation site Tequatrovirus T4
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.72 S-adenosyl-L-methionine + DNA adenine hemimethylated DNA Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?
2.1.1.72 S-adenosyl-L-methionine + DNA adenine binding site is the GATC sequence, the adenine is located in the palindromic recognition site GATC, substrate binding mechanism Tequatrovirus T4 S-adenosyl-L-homocysteine + DNA 6-methylaminopurine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.72 monomer
-
 Tequatrovirus T4
2.1.1.72 More enzyme is predominantly a monomer in solution, mechanism of DNA-induced dimerization upon binding to DNA substrate and complex formation, structure and interaction analysis of the enzyme complexed with a 12mer DNA duplex and S-adenosyl-L-homocysteine in a enzyme/duplex ratio of 2, no flipping of adenine out of the DNA duplex Tequatrovirus T4

Synonyms

EC Number Synonyms Comment Organism
2.1.1.72 More enzyme belongs to the Dam MTase family Tequatrovirus T4
2.1.1.72 T4 Dam DNA methyltransferase
-
 Tequatrovirus T4
2.1.1.72 T4Dam
-
 Tequatrovirus T4