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Literature summary extracted from

  • Rendon, J.L.; del Arenal, I.P.; Guevara-Flores, A.; Uribe, A.; Plancarte, A.; Mendoza-Hernandez, G.
    Purification, characterization and kinetic properties of the multifunctional thioredoxin-glutathione reductase from Taenia crassiceps metacestode (cysticerci) (2004), Mol. Biochem. Parasitol., 133, 61-69.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.8.1.B1 continuous freezing and thawing results in loss of the enzyme activity Taenia crassiceps

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.B1 auranofin
-
 Taenia crassiceps
1.8.1.7 auranofin complete inhibition of both enzyme reductase activities at 10 nM Taenia crassiceps
1.8.1.9 auranofin
-
 Taenia crassiceps

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.B1 0.003
-
 NADPH pH 7.8, 25°C Taenia crassiceps
1.8.1.B1 0.015
-
 GSSG pH 7.8, 25°C Taenia crassiceps
1.8.1.B1 0.017
-
 thioredoxin pH 7.8, 25°C Taenia crassiceps
1.8.1.B1 0.049
-
 5,5'-dithiobis(2-nitrobenzoic acid) pH 7.8, 25°C Taenia crassiceps
1.8.1.7 additional information
-
 additional information kinetics, hysteretic behaviour Taenia crassiceps
1.8.1.7 0.003
-
 NADPH pH 7.8, 25°C Taenia crassiceps
1.8.1.7 0.015
-
 glutathione disulfide pH 7.8, 25°C Taenia crassiceps
1.8.1.9 0.003
-
 NADPH
-
 Taenia crassiceps
1.8.1.9 0.015
-
 GSSG
-
 Taenia crassiceps
1.8.1.9 0.017
-
 thioredoxin disulfide
-
 Taenia crassiceps
1.8.1.9 0.049
-
 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Taenia crassiceps

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.1.7 soluble
-
 Taenia crassiceps
-
-
1.8.1.9 soluble
-
 Taenia crassiceps
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.1.9 selenium contains an essential selenocysteine residue Taenia crassiceps

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.B1 66000
-
 1 * 66000, gel electrophoresis under denaturing conditions Taenia crassiceps
1.8.1.B1 136000
-
 gel filtration Taenia crassiceps
1.8.1.7 65000
-
 2 * 65000, SDS-PAGE Taenia crassiceps
1.8.1.7 136000
-
 gel filtration Taenia crassiceps
1.8.1.9 66000
-
 2 * 66000, SDS-PAGE Taenia crassiceps
1.8.1.9 136000
-
 gel filtration Taenia crassiceps

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.7 glutathione disulfide + NADPH Taenia crassiceps
-
 glutathione + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.B1 Taenia crassiceps
-
-
-
1.8.1.7 Taenia crassiceps
-
 parasite isolated from mice, enzyme is a multifunctional thioredoxin-glutathione reductase TGR
-
1.8.1.9 Taenia crassiceps
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.B1
-
 Taenia crassiceps
1.8.1.7 647fold to homogeneity from metacystodes by ammonium sulfate fractionation, ion exchange chromatography, affinity chromatography, and gel filtration Taenia crassiceps
1.8.1.9
-
 Taenia crassiceps

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.1.7 2 glutathione + NADP+ = glutathione disulfide + NADPH + H+ the multifunctional enzyme possesses both thioredoxin reductase activity, EC 1.8.1.9, glutathione reductase activity, EC 1.8.1.7, and glutaredoxin activity Taenia crassiceps
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.7 metacestode i.e. cysticeri, recovered from peritoneal cavity of 6-8 months before inoculated female Balb/c mice Taenia crassiceps
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.B1 4.7
-
 GSSG + NADPH Taenia crassiceps
1.8.1.B1 10.3
-
 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH Taenia crassiceps
1.8.1.B1 17.5
-
 thioredoxin disulfide + NADPH Taenia crassiceps
1.8.1.7 4.9
-
 purified enzyme, substrate glutathione disulfide Taenia crassiceps
1.8.1.9 10.3
-
 GSSG + NADPH Taenia crassiceps
1.8.1.9 17.5
-
 thioredoxin disulfide + NADPH Taenia crassiceps

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.B1 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ absolute specificity for NADPH as electron donor Taenia crassiceps 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.B1 GSSG + NADPH + H+ absolute specificity for NADPH as electron donor Taenia crassiceps glutathione + NADP+
-
 ?
1.8.1.B1 thioredoxin disulfide + NADPH absolute specificity for NADPH as electron donor Taenia crassiceps thioredoxin + NADP+
-
 ?
1.8.1.7 glutathione disulfide + NADPH
-
 Taenia crassiceps glutathione + NADP+
-
 ?
1.8.1.9 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
-
 Taenia crassiceps 2-nitro-5-thiobenzoate + NADP+
-
 ?
1.8.1.9 GSSG + NADPH
-
 Taenia crassiceps GSH + NADP+
-
 ?
1.8.1.9 thioredoxin disulfide + NADPH
-
 Taenia crassiceps thioredoxin + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.1.B1 dimer 1 * 66000, gel electrophoresis under denaturing conditions Taenia crassiceps
1.8.1.7 dimer 2 * 65000, SDS-PAGE Taenia crassiceps
1.8.1.9 dimer 2 * 66000, SDS-PAGE Taenia crassiceps

Synonyms

EC Number Synonyms Comment Organism
1.8.1.B1 thioredoxin-glutathione reductase
-
 Taenia crassiceps
1.8.1.7 multifunctional thioredoxin-glutathione reductase
-
 Taenia crassiceps
1.8.1.7 TGR
-
 Taenia crassiceps
1.8.1.9 multifunctional thioredoxin-glutathione reductase
-
 Taenia crassiceps
1.8.1.9 TGR
-
 Taenia crassiceps

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.8.1.7 25
-
 assay at Taenia crassiceps

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.B1 0.17
-
 5,5'-dithiobis(2-nitrobenzoic acid) pH 7.8, 25°C Taenia crassiceps
1.8.1.B1 5.1
-
 GSSG pH 7.8, 25°C Taenia crassiceps
1.8.1.B1 19.2
-
 thioredoxin pH 7.8, 25°C Taenia crassiceps
1.8.1.9 5.1
-
 GSSG
-
 Taenia crassiceps
1.8.1.9 19.2
-
 thioredoxin disulfide
-
 Taenia crassiceps

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.7 7.8
-
 assay at Taenia crassiceps

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.7 FAD 1.2 mol per mol of enzyme subunit bound Taenia crassiceps
1.8.1.7 NADPH
-
 Taenia crassiceps
1.8.1.9 FAD contains 1.2 mol per mol of enzyme subunit Taenia crassiceps
1.8.1.9 NADPH
-
 Taenia crassiceps

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.8.1.B1 Taenia crassiceps
-
-
 5.2
1.8.1.7 Taenia crassiceps isoelectric focusing
-
 5.2
1.8.1.9 Taenia crassiceps
-
-
 5.2