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Literature summary extracted from

  • Miyazaki, T.; Miyazaki, J.; Yamane, H.; Nishiyama, M.
    alpha-Aminoadipate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus (2004), Microbiology, 150, 2327-2334.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.39
-
Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.39 additional information enzyme disruption mutant, needs longer lag phase for growth and shows slower growth in minimal medium. Addition of alpha-aminoadipate or lysine shortens the lag phase and improves growth rate Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.39 0.0133
-
2-oxoisovalerate pH 7.5, 45°C Thermus thermophilus
2.6.1.39 0.024
-
2-oxoadipate pH 7.5, 45°C Thermus thermophilus
2.6.1.39 0.0288
-
2-oxoisocaproate pH 7.5, 45°C Thermus thermophilus
2.6.1.39 0.1483
-
2-oxo-3-methylvalerate pH 7.5, 45°C Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.39 additional information
-
sedimentation equilibrium analysis, equilibrium constant 2.4 + 10-5 M Thermus thermophilus
2.6.1.39 43845
-
2 * 43845, calculated, 2 * 44000, SDS-PAGE Thermus thermophilus
2.6.1.39 44000
-
2 * 43845, calculated, 2 * 44000, SDS-PAGE Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.39 Thermus thermophilus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.39 513
-
substrate phenylpyruvate, pH 7.5, 45°C Thermus thermophilus
2.6.1.39 561
-
substrate 2-oxo-3-methylvalerate, pH 7.5, 45°C Thermus thermophilus
2.6.1.39 568
-
substrate pyruvate, pH 7.5, 45°C Thermus thermophilus
2.6.1.39 661
-
substrate oxaloacetate, pH 7.5, 45°C Thermus thermophilus
2.6.1.39 1102
-
substrate 2-oxoisovalerate, pH 7.5, 45°C Thermus thermophilus
2.6.1.39 5079
-
substrate 2-oxoisocaproate, pH 7.5, 45°C Thermus thermophilus
2.6.1.39 6788
-
substrate 2-oxoadipate, pH 7.5, 45°C Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.39 2-oxo-3-methylvalerate + L-glutamate
-
Thermus thermophilus 2-amino-3-methylpentanoate + 2-oxoglutarate
-
?
2.6.1.39 2-oxoadipate + L-glutamate
-
Thermus thermophilus L-2-aminoadipate + 2-oxoglutarate
-
?
2.6.1.39 2-oxoisocaproate + L-glutamate
-
Thermus thermophilus L-leucine + 2-oxoglutarate
-
?
2.6.1.39 2-oxoisovalerate + L-glutamate
-
Thermus thermophilus L-valine + 2-oxoglutarate
-
?
2.6.1.39 oxaloacetate + L-glutamate
-
Thermus thermophilus 2-aminosuccinate + 2-oxoglutarate
-
?
2.6.1.39 phenylpyruvate + 2-aminoadipate
-
Thermus thermophilus L-phenylalanine + 2-oxoglutarate
-
?
2.6.1.39 pyruvate + L-glutamate
-
Thermus thermophilus L-alanine + 2-oxoglutarate
-
?

Subunits

EC Number Subunits Comment Organism
2.6.1.39 dimer 2 * 43845, calculated, 2 * 44000, SDS-PAGE Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.39 2.1
-
2-oxoisovalerate pH 7.5, 45°C Thermus thermophilus
2.6.1.39 3.5
-
2-oxo-3-methylvalerate pH 7.5, 45°C Thermus thermophilus
2.6.1.39 17.8
-
2-oxoisocaproate pH 7.5, 45°C Thermus thermophilus
2.6.1.39 22.3
-
2-oxoadipate pH 7.5, 45°C Thermus thermophilus