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Literature summary extracted from
- Multiple methionine sulfoxide reductase genes in Staphylococcus aureus: expression of activity and roles in tolerance of oxidative stress (2003), Microbiology, 149, 2739-2747.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.4.11 | complementation of the msrA1/msrA2 double mutant with the msr1 construct, promotor exchange between msrA1 and msrA2 | Staphylococcus aureus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.8.4.11 | additional information | construction of a msrA2 knockout mutant, a msrA1 knockout mutant, and a msrA1/msrA2 double knockout mutant, the msrA2 mutant strain and the msrA1/msrA2 double mutant strain show H2O2 tolerance like the wild-type parent strain, but the double mutant strain complemented by msrA2 is H2O2 susceptible, effects of H2O2 or oxidative stress are related to the promotors, overview | Staphylococcus aureus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | Staphylococcus aureus | MsrAs are specific for the (S)-form of the substrate | L-methionine + thioredoxin disulfide + H2O | - |
? |  |
| 1.8.4.11 | additional information | Staphylococcus aureus | the MsrA1/MsrB system is physiologically more significant in Staphylococcus aureus than MsrA2 | ? | - |
? | |
| 1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | Staphylococcus aureus | MsrB is specific for the (R)-form of the substrate | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | additional information | Staphylococcus aureus | the MsrA1/MsrB system is physiologically more significant in Staphylococcus aureus than MsrA2 | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.4.11 | Staphylococcus aureus | - |
strains RN450 and BB270, and derivatives, 3 genes msrA1, msrA2, and msrA3 encoding MsrA enzyme forms | - |
| 1.8.4.12 | Staphylococcus aureus | - |
strains RN450 and BB270, and derivatives, 1 gene msrB | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | additional information | - |
69% of wild-type strain RB450 MsrA activity belongs to MsrA1, 8% to MsrA2, and 23% to MsrA3 | Staphylococcus aureus |
| 1.8.4.11 | 0.00006 | - |
msrA1/msrA2 double knockout strain RN450 | Staphylococcus aureus |
| 1.8.4.11 | 0.00006 | - |
msrA1/msrA2 double knockout strain RN450, substrate L-methionine (S)-sulfoxide | Staphylococcus aureus |
| 1.8.4.11 | 0.00008 | - |
msrA1 knockout strain RN450 | Staphylococcus aureus |
| 1.8.4.11 | 0.00008 | - |
msrA1 knockout strain RN450, substrate L-methionine (S)-sulfoxide | Staphylococcus aureus |
| 1.8.4.11 | 0.00024 | - |
msrA2 knockout strain RN450 | Staphylococcus aureus |
| 1.8.4.11 | 0.00024 | - |
msrA2 knockout strain RN450, substrate L-methionine (S)-sulfoxide | Staphylococcus aureus |
| 1.8.4.11 | 0.00026 | - |
wild-type strain RN450 | Staphylococcus aureus |
| 1.8.4.11 | 0.00026 | - |
wild-type strain RN450, substrate L-methionine (S)-sulfoxide | Staphylococcus aureus |
| 1.8.4.12 | 0.000029 | - |
wild-type strain RN450, substrate L-methionine (R)-sulfoxide | Staphylococcus aureus |
| 1.8.4.12 | 0.00003 | - |
msrA2 knockout strain RN450, substrate L-methionine (R)-sulfoxide | Staphylococcus aureus |
| 1.8.4.12 | 0.0012 | - |
msrA1 knockout strain RN450, substrate L-methionine (R)-sulfoxide | Staphylococcus aureus |
| 1.8.4.12 | 0.0015 | - |
msrA1/msrA2 double knockout strain RN450, substrate L-methionine (R)-sulfoxide | Staphylococcus aureus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.11 | L-methionine (S)-sulfoxide + thioredoxin | MsrAs are specific for the (S)-form of the substrate | Staphylococcus aureus | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.11 | additional information | the MsrA1/MsrB system is physiologically more significant in Staphylococcus aureus than MsrA2 | Staphylococcus aureus | ? | - |
? | |
| 1.8.4.12 | L-methionine (R)-sulfoxide + thioredoxin | MsrB is specific for the (R)-form of the substrate | Staphylococcus aureus | L-methionine + thioredoxin disulfide + H2O | - |
? | |
| 1.8.4.12 | additional information | the MsrA1/MsrB system is physiologically more significant in Staphylococcus aureus than MsrA2 | Staphylococcus aureus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.4.11 | methionine sulfoxide reductase | - |
Staphylococcus aureus |
| 1.8.4.12 | methionine sulfoxide reductase | - |
Staphylococcus aureus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 37 | - |
assay at | Staphylococcus aureus |
| 1.8.4.12 | 37 | - |
assay at | Staphylococcus aureus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.11 | 7.5 | - |
assay at | Staphylococcus aureus |
| 1.8.4.12 | 7.5 | - |
assay at | Staphylococcus aureus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.11 | thioredoxin | - |
Staphylococcus aureus | |
| 1.8.4.12 | thioredoxin | - |
Staphylococcus aureus | |
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Release 2023.1 (January 2023)
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