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Literature summary extracted from

  • Laska, S.; Lottspeich, F.; Kletzin, A.
    Membrane-bound hydrogenase and sulfur reductase of the hyperthermophilic and acidophilic archaeon Acidianus ambivalens (2003), Microbiology, 149, 2357-2371.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.98.4 the SR gene cluster encompasses five ORFs with a total length of 7389 bp. The sreA gene encodes the 110000 Da subunit of SR. sreB encodes an FeS protein, sreC encodes a hydrophobic protein with 10 predicted transmembrane helices. The sreD gene encodes a putative hydrophilic FeS protein with 26 cysteine residues and of unknown cluster composition. The sreE gene encodes a protein with similarity to reductase assembly proteins Acidianus ambivalens
1.12.99.6
-
 Acidianus ambivalens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.98.4 membrane bound to Acidianus ambivalens 16020
-
1.12.99.6 membrane membrane bound Acidianus ambivalens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.99.6 Fe 0.134 mmol Fe per g protein Acidianus ambivalens
1.12.99.6 Mo 0.0025 mmol Mo per g protein Acidianus ambivalens
1.12.99.6 Ni 0.0025 mmol Ni per g protein Acidianus ambivalens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.99.6 41000
-
 x * 41000 + x * 66000, SDS-PAGE Acidianus ambivalens
1.12.99.6 66000
-
 x * 41000 + x * 66000, SDS-PAGE Acidianus ambivalens

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.4 Acidianus ambivalens
-
-
-
1.12.99.6 Acidianus ambivalens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.98.4
-
 Acidianus ambivalens
1.12.99.6 density-gradient centrifugation, column chromatography Acidianus ambivalens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.99.6 114
-
 acceptor methyl viologen Acidianus ambivalens
1.12.99.6 624
-
 acceptor 2,3-dimethylnaphthoquinol Acidianus ambivalens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.4 sulfur + H2 in solubilized membrane fraction no activity is detected without the addition of electron carriers: 2,3-dimethylnaphthoquinone, plastoquinone or horse heart cytochrome c Acidianus ambivalens H2S
-
 ?
1.12.99.6 H2 + oxidized 2,3-dimethylnaphthoquinone
-
 Acidianus ambivalens 2,3-dimethylnaphthoquinol
-
 ?
1.12.99.6 H2 + oxidized benzyl viologen
-
 Acidianus ambivalens reduced benzyl viologen + H+
-
 ?
1.12.99.6 H2 + oxidized methyl viologen
-
 Acidianus ambivalens reduced methyl viologen + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.99.6 ? x * 41000 + x * 66000, SDS-PAGE Acidianus ambivalens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.98.4 8
-
 no activity is observed below pH 6.0, the activity gradually increases up to pH 8.0, above pH 8.0 the rate of non-enzymic sulfur disproportionation reaction is higher than the enzymic rate Acidianus ambivalens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.12.98.4 6 8 no activity is observed below pH 6.0, the activity gradually increases up to pH 8.0, above pH 8.0 the rate of non-enzymic sulfur disproportionation reaction is higher than the enzymic rate Acidianus ambivalens

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.98.4 NADPH addition increases activity Acidianus ambivalens