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Literature summary extracted from

  • Wendeler, M.; Reilaender, H.; Hoernschemeyer, J.; Schwarzmann, G.; Kolter, T.; Sandhoff, K.
    Recombinant ganglioside GM2 synthase-expression in insect cells and enzyme assay (2003), Methods Enzymol., 363, 476-489.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.92 synthesis expression of enzyme both as complete membrane-bound enzyme and as a soluble form in the baculovirus insect cell expression system Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.92 additional information
-
 additional information assay method Mus musculus
2.4.1.92 0.097
-
 UDP-GalNAc pH 7.3, 37°C, soluble domain Mus musculus
2.4.1.92 0.19
-
 UDP-GalNAc pH 7.3, 37°C, membrane-bound complete enzyme Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.92 membrane
-
 Mus musculus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.92 60000
-
 2 * 60000, SDS-PAGE Mus musculus
2.4.1.92 120000
-
 PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.92 Mus musculus
-
 expression in baculovirus system, membrane-bound complete enzyme and soluble domain
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.92 53.3
-
 pH 7.3, 37°C, soluble domain Mus musculus
2.4.1.92 4566
-
 pH 7.3, 37°C, membrane-bound complete enzyme Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.92 UDP-GalNAc + n-octyl-beta-D-glucopyranoside
-
 Mus musculus UDP + GalNAcbeta(1-4)-glc-n-octyl
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.92 dimer 2 * 60000, SDS-PAGE Mus musculus