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Literature summary extracted from

  • Jinno, H.; Saeki, M.; Saito, Y.; Tanaka-Kagawa, T.; Hanioka, N.; Sai, K.; Kaniwa, N.; Ando, M.; Shirao, K.; Minami, H.; Ohtsu, A.; Yoshida, T.; Saijo, N.; Ozawa, S.; Sawada, J.
    Functional characterization of human UDP-glucuronosyltransferase 1A9 variant, D256N, found in Japanese cancer patients (2003), J. Pharmacol. Exp. Ther., 306, 688-693.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.17 expression of wild-type and mutant D256N enzymes in COS-1 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.17 additional information mutant isozyme UGT1A9 D256N is isolated from a japanese cancer patient, site-directed mutagenesis for expression plasmid construction, the mutant shows less than 5% of wild-type isozyme activity with 7-ethyl-10-hydroxycampothecin as substrate, the naturally occuring mutant isozyme might be involved in irinotecan metabolism in vivo Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.17 19.3
-
 7-ethyl-10-hydroxycampothecin recombinant wild-type isozyme UGT1A9, pH.4, 37°C Homo sapiens
2.4.1.17 44.4
-
 7-ethyl-10-hydroxycampothecin recombinant UGT1A9 mutant D256N isozyme, pH.4, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.17 membrane
-
 Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.17 Mg2+
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.17 Homo sapiens O60656 isozymes UGT1A1, UGT1A7, UGT1A9, naturally occuring UGT1A9 mutant D256N, the latter from a japanese cancer patient
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.17 liver isozyme UGT1A1 and UGT1A9 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.17 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.17 additional information in contrast to isozymes UGT1A1 and UGT1A7, the isozyme UGT1A9 does not show polymorphism influencing the glucuronidation activity of SN-38 Homo sapiens ?
-
 ?
2.4.1.17 UDP-glucuronate + 7-ethyl-10-hydroxycampothecin i.e. SN-38, isozymes UGT1A1, UGT1A7, and UGT1A9 Homo sapiens UDP + 7-ethyl-10-hydroxycampothecin 1-O-beta-D-glucuronide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.17 UDP-glucuronosyltransferase 1A9
-
 Homo sapiens
2.4.1.17 UGT1A9
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.17 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.17 7.4
-
 assay at Homo sapiens