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Literature summary extracted from

  • Choi, B.H.; Chae, H.D.; Park, T.J.; Oh, J.; Lim, J.; Kang, S.S.; Ha, H.; Kim, K.T.
    Protein kinase C regulates the activity and stability of serotonin N-acetyltransferase (2004), J. Neurochem., 90, 442-454.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.87 forskolin decreases KM-value for tryptamine by 32% Rattus norvegicus
2.3.1.87 phorbol 12-myristate 13-acetate decreases KM-value for tryptamine by 44% Rattus norvegicus
2.7.11.13 phorbol 12-myristate 13-acetate activates PKC 4fold Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.87 S192V decrease in Km-value obtained by treatment with phorbol 12-myristate 13-acetate or forskolin is similar to the decrease observed in wild-type cells Rattus norvegicus
2.3.1.87 T127V decrease in Km-value obtained by treatment with phorbol 12-myristate 13-acetate or forskolin is similar to the decrease observed in wild-type cells Rattus norvegicus
2.3.1.87 T29V mutant enzyme shows an increase in Km-value obtained by treatment with phorbol 12-myristate 13-acetate or forskolin, decrease in Km-value is observed in wild-type cells Rattus norvegicus
2.3.1.87 T29V/S203G mutant enzyme shows an increase in Km-value obtained by treatment with phorbol 12-myristate 13-acetate or forskolin, decrease in Km-value is observed in wild-type cells Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.13 GF109203X specific inhibition of PKC Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.87 0.291
-
tryptamine pH 6.8, 37°C, mutant enzyme S192V Rattus norvegicus
2.3.1.87 0.317
-
tryptamine pH 6.8, 37°C, mutant enzyme T29V/S203G Rattus norvegicus
2.3.1.87 0.344
-
tryptamine pH 6.8, 37°C, mutant enzyme T127V Rattus norvegicus
2.3.1.87 0.361
-
tryptamine pH 6.8, 37°C, wild-type enzyme Rattus norvegicus
2.3.1.87 0.413
-
tryptamine pH 6.8, 37°C, mutant enzyme T29V Rattus norvegicus
2.3.1.87 0.417
-
tryptamine pH 6.8, 37°C, mutant enzyme S203G Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.13 Ca2+
-
Rattus norvegicus
2.7.11.13 Mg2+
-
Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.13 ATP + serotonin N-acetyltransferase Rattus norvegicus PKC regulates the activity and stability of serotonin N-acetyltransferase in vivo, recombinant enzyme expressed in COS-7 cells ADP + phosphorylated serotonin N-acetyltransferase
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.87 Rattus norvegicus
-
-
-
2.7.11.13 Rattus norvegicus
-
male sprague-dawley rats
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.87 phosphoprotein phosphorylated at Thr29 by protein kinase C activation through the alpha1-adrenergic receptor in rat pineal glands. Phosphorylation may contribute to the stability and activity of the enzyme Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.87 pineal gland
-
Rattus norvegicus
-
2.7.11.13 pineal gland
-
Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.87 acetyl-CoA + tryptamine
-
Rattus norvegicus CoA + N-acetyltryptamine
-
?
2.7.11.13 ATP + serotonin N-acetyltransferase PKC regulates the activity and stability of serotonin N-acetyltransferase in vivo, recombinant enzyme expressed in COS-7 cells Rattus norvegicus ADP + phosphorylated serotonin N-acetyltransferase
-
?
2.7.11.13 ATP + serotonin N-acetyltransferase recombinant enzyme expressed in COS-7 cells, phosphorylation at Thr29 Rattus norvegicus ADP + phosphorylated serotonin N-acetyltransferase
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.87 AANAT
-
Rattus norvegicus
2.3.1.87 serotonin N-acetyltransferase
-
Rattus norvegicus
2.7.11.13 PKC
-
Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.13 30
-
assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.13 7.4
-
assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.13 ATP
-
Rattus norvegicus