EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.87 | forskolin | decreases KM-value for tryptamine by 32% | Rattus norvegicus | |
2.3.1.87 | phorbol 12-myristate 13-acetate | decreases KM-value for tryptamine by 44% | Rattus norvegicus | |
2.7.11.13 | phorbol 12-myristate 13-acetate | activates PKC 4fold | Rattus norvegicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.87 | S192V | decrease in Km-value obtained by treatment with phorbol 12-myristate 13-acetate or forskolin is similar to the decrease observed in wild-type cells | Rattus norvegicus |
2.3.1.87 | T127V | decrease in Km-value obtained by treatment with phorbol 12-myristate 13-acetate or forskolin is similar to the decrease observed in wild-type cells | Rattus norvegicus |
2.3.1.87 | T29V | mutant enzyme shows an increase in Km-value obtained by treatment with phorbol 12-myristate 13-acetate or forskolin, decrease in Km-value is observed in wild-type cells | Rattus norvegicus |
2.3.1.87 | T29V/S203G | mutant enzyme shows an increase in Km-value obtained by treatment with phorbol 12-myristate 13-acetate or forskolin, decrease in Km-value is observed in wild-type cells | Rattus norvegicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.13 | GF109203X | specific inhibition of PKC | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.87 | 0.291 | - |
tryptamine | pH 6.8, 37°C, mutant enzyme S192V | Rattus norvegicus | |
2.3.1.87 | 0.317 | - |
tryptamine | pH 6.8, 37°C, mutant enzyme T29V/S203G | Rattus norvegicus | |
2.3.1.87 | 0.344 | - |
tryptamine | pH 6.8, 37°C, mutant enzyme T127V | Rattus norvegicus | |
2.3.1.87 | 0.361 | - |
tryptamine | pH 6.8, 37°C, wild-type enzyme | Rattus norvegicus | |
2.3.1.87 | 0.413 | - |
tryptamine | pH 6.8, 37°C, mutant enzyme T29V | Rattus norvegicus | |
2.3.1.87 | 0.417 | - |
tryptamine | pH 6.8, 37°C, mutant enzyme S203G | Rattus norvegicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.13 | Ca2+ | - |
Rattus norvegicus | |
2.7.11.13 | Mg2+ | - |
Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.11.13 | ATP + serotonin N-acetyltransferase | Rattus norvegicus | PKC regulates the activity and stability of serotonin N-acetyltransferase in vivo, recombinant enzyme expressed in COS-7 cells | ADP + phosphorylated serotonin N-acetyltransferase | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.87 | Rattus norvegicus | - |
- |
- |
2.7.11.13 | Rattus norvegicus | - |
male sprague-dawley rats | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.3.1.87 | phosphoprotein | phosphorylated at Thr29 by protein kinase C activation through the alpha1-adrenergic receptor in rat pineal glands. Phosphorylation may contribute to the stability and activity of the enzyme | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.1.87 | pineal gland | - |
Rattus norvegicus | - |
2.7.11.13 | pineal gland | - |
Rattus norvegicus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.87 | acetyl-CoA + tryptamine | - |
Rattus norvegicus | CoA + N-acetyltryptamine | - |
? | |
2.7.11.13 | ATP + serotonin N-acetyltransferase | PKC regulates the activity and stability of serotonin N-acetyltransferase in vivo, recombinant enzyme expressed in COS-7 cells | Rattus norvegicus | ADP + phosphorylated serotonin N-acetyltransferase | - |
? | |
2.7.11.13 | ATP + serotonin N-acetyltransferase | recombinant enzyme expressed in COS-7 cells, phosphorylation at Thr29 | Rattus norvegicus | ADP + phosphorylated serotonin N-acetyltransferase | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.87 | AANAT | - |
Rattus norvegicus |
2.3.1.87 | serotonin N-acetyltransferase | - |
Rattus norvegicus |
2.7.11.13 | PKC | - |
Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.11.13 | 30 | - |
assay at | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.11.13 | 7.4 | - |
assay at | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.13 | ATP | - |
Rattus norvegicus |