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Literature summary extracted from

  • Petri, A.; Gambicorti, T.; Salvadori, P.
    Covalent immobilization of chloroperoxidase on silica gel and properties of the immobilized biocatalyst (2004), J. Mol. Catal. B, 27, 103-106.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.11.1.10 immobilization of the enzyme on silica gel enhances the stability with respect to the effect of pH and oxidizing agent concentrations Leptoxyphium fumago

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.10 Leptoxyphium fumago
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.11.1.10 tert-butyl hydroperoxide, 10 mM, soluble enzyme is almost completely inactive after 2 h, immobilized enzyme still retains 20% of its activity after 4 h. After 24 h in 0.1 mM tert-butyl hydroperoxide the activity of immobilized enzyme is unaffected, while soluble enzyme loses more than 50% of its original activity Leptoxyphium fumago

Storage Stability

EC Number Storage Stability Organism
1.11.1.10 -20°C, pH 6, less than 20% loss of activity after 6 months Leptoxyphium fumago

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.10 monochlorodimedone + Cl- + H2O2
-
 Leptoxyphium fumago dichlorodimedone + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.10 chloroperoxidase
-
 Leptoxyphium fumago
1.11.1.10 CPO
-
 Leptoxyphium fumago

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.10 4 6 soluble and immobilized enzyme Leptoxyphium fumago

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.10 3.7 7 pH 3.7: about 90% of maximal activity, soluble and immobilized enzyme. pH 7.0: about 55% of maximal activity of immobilized enzyme, about 50% of maximal activity of soluble enzyme Leptoxyphium fumago

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.10 additional information
-
 immobilization of the enzyme on silica gel enhances the stability with respect to the effect of pH and oxidizing agent concentrations Leptoxyphium fumago
1.11.1.10 2.8 6 25°C, 24 h, immobilized and soluble enzyme are stable Leptoxyphium fumago
1.11.1.10 7
-
 25°C, 24 h, about 50% loss of activity of soluble enzyme, about 40% loss of activity of immobilized enzyme Leptoxyphium fumago