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Literature summary extracted from

  • Musayev, F.; Sachdeva, S.; Scarsdale, J.N.; Reynolds, K.A.; Wright, H.T.
    Crystal structure of a substrate complex of Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthase III (FabH) with lauroyl-coenzyme A (2005), J. Mol. Biol., 346, 1313-1321.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.41 in complex with lauroyl-CoA Mycobacterium tuberculosis
2.3.1.180 purified recombinant enzyme in complex with lauroyl-CoA, X-ray diffraction structure determination and analysis at 1.85-2.3 A resolution, modeling Mycobacterium tuberculosis
2.3.1.301 structure of mutant C112A with lauroyl-coenzyme A. An elongated channelextending from the mutated active-site cysteine defines the acyl group binding locus. CoA lies in a second channel, bound primarily through interactions of its nucleotide group at the enzyme surface Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.301 C112A inactive Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] Mycobacterium tuberculosis the enzyme initiates the fatty acid biosynthesis acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] Mycobacterium tuberculosis H37Rv the enzyme initiates the fatty acid biosynthesis acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] Mycobacterium tuberculosis ATCC 25618 the enzyme initiates the fatty acid biosynthesis acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.41 Mycobacterium tuberculosis
-
-
-
2.3.1.180 Mycobacterium tuberculosis P9WNG3
-
-
2.3.1.180 Mycobacterium tuberculosis H37Rv P9WNG3
-
-
2.3.1.301 Mycobacterium tuberculosis P9WNG3
-
-
2.3.1.301 Mycobacterium tuberculosis ATCC 25618 P9WNG3
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.180 acetyl-CoA + a malonyl-[acyl-carrier protein] = an acetoacetyl-[acyl-carrier protein] + CoA + CO2 catalytic reaction mechanism for decarboxylation and condensation Mycobacterium tuberculosis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.41 malonyl-[acyl-carrier protein] + lauroyl-CoA
-
 Mycobacterium tuberculosis ? + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 malonyl-[acyl-carrier protein] + myristoyl-CoA
-
 Mycobacterium tuberculosis ? + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 malonyl-[acyl-carrier protein] + palmitoyl-CoA
-
 Mycobacterium tuberculosis ? + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] the enzyme initiates the fatty acid biosynthesis Mycobacterium tuberculosis acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein]
-
 Mycobacterium tuberculosis acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] the enzyme initiates the fatty acid biosynthesis Mycobacterium tuberculosis H37Rv acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein]
-
 Mycobacterium tuberculosis H37Rv acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] the enzyme initiates the fatty acid biosynthesis Mycobacterium tuberculosis ATCC 25618 acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein]
-
 Mycobacterium tuberculosis ATCC 25618 acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 lauroyl-CoA + malonyl-[acyl-carrier protein] the primer substrate lauroyl-CoA is bound to subunit A, binding structure, lauroyl is bound in a channel Mycobacterium tuberculosis 3-oxo-myristoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 lauroyl-CoA + malonyl-[acyl-carrier protein] the primer substrate lauroyl-CoA is bound to subunit A, binding structure, lauroyl is bound in a channel Mycobacterium tuberculosis H37Rv 3-oxo-myristoyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 lauroyl-CoA + malonyl-[acyl-carrier protein] the primer substrate lauroyl-CoA is bound to subunit A, binding structure, lauroyl is bound in a channel Mycobacterium tuberculosis ATCC 25618 3-oxo-myristoyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.180 dimer dimer packing analysis from crystal structure Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.3.1.180 beta-ketoacyl-acyl carrier protein synthase III
-
 Mycobacterium tuberculosis
2.3.1.180 FabH
-
 Mycobacterium tuberculosis
2.3.1.301 FabH
-
 Mycobacterium tuberculosis
2.3.1.301 Rv0533c
-
 Mycobacterium tuberculosis